Rhodamine b isothiocyanate rbitc

Manufactured by Merck Group

Sourced in United States

Rhodamine B isothiocyanate (RBITC) is a fluorescent dye used in various research applications. It is a derivative of the rhodamine B compound, which exhibits strong absorption and emission properties. RBITC is commonly used for labeling and detection purposes in biological and analytical techniques, such as fluorescence microscopy, flow cytometry, and protein labeling.

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7 protocols using rhodamine b isothiocyanate rbitc

Simvastatin-Chitosan Nanoparticles for Drug Delivery

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The simvastatin (SV) used in this study was a generous gift from the Riyadh Pharma Co., Saudi Arabia. Low-molecular-weight chitosan (viscosity 20,000 cps) (degree of deacetylation [DD] of 92%), chondroitin 4-sulfate sodium salt (ChS, sodium content 5.6% w/w, molecular weight 59 kDa), simvastatin hydroxy acid ammonium salt sodium (SVA), lovastatin, lovastatin hydroxy acid sodium salt, rhodamine B isothiocyanate (RBITC), and carboxymethylcellulose were purchased from Sigma Aldrich Chemical Co. Ltd. (St. Louis, MO, USA). Sodium tripolyphosphate (TPP) and glacial acetic acid were obtained from BDH Organic (Poole, Dorset, UK). Ethanol, methanol, acetonitrile, and phosphoric acid (HPLC grade) were obtained from Fisher Scientific Co., (Loughborough, UK). phosphate buffer solution (PBS) (pH 7.4) solution (8 g NaCl (137 mM), 0.2 g KCl (2.7 mM), 1.15 g Na₂HPO4··7H₂O (8.1 mM), and 0.2 g KH₂PO₄ (1.47 mM) in 1000 mL deionized water. Deionized water was obtained using a Milli-Q® water purifier (Millipore, France) and used throughout the study.

Faris T.M., Harisa G.I., Alanazi F.K., Samy A.M, & Nasr F.A. (2020). Developed simvastatin chitosan nanoparticles co-crosslinked with tripolyphosphate and chondroitin sulfate for ASGPR-mediated targeted HCC delivery with enhanced oral bioavailability. Saudi Pharmaceutical Journal : SPJ, 28(12), 1851-1867.

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Lactobacillus acidophilus 11073 Encapsulation

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Lactobacillus acidophilus 11073 was obtained from Gaofuji Biological Technology Co., Ltd. (Chengdu, China). Rice starch (amylose content of 21.54 ± 0.85%) was obtained from Haixi Biological Technology Co., Ltd. (Guangzhou, China). WPI was obtained from Haofa Biological Technology Co., Ltd. (Zhengzhou, China). De Man, Rogosa Sharp (MRS) agar and broth were obtained from Aoboxing Biological Technology Co., Ltd. (Beijing, China). OSA, pepsin (P7012), pancreatin (P7545), amyloglucosidase (A7095), porcine-bile extract (B8613) and rhodamine B isothiocyanate (RBITC) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Other analytical pure chemical reagents were obtained from Cologne Chemical Co. (Chengdu, China).

Liu Q., Lin C., Yang X., Wang S., Yang Y., Liu Y., Xiong M., Xie Y., Bao Q, & Yuan Y. (2023). Improved Viability of Probiotics via Microencapsulation in Whey-Protein-Isolate-Octenyl-Succinic-Anhydride-Starch-Complex Coacervates. Molecules, 28(15), 5732.

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Functionalized Platinum Nanocomposite Synthesis

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Tetraammine platinum (II) chloride salt [Pt(NH₃)₄]Cl₂⋅H₂O, poly(ethylene glycol) diamine (H₂N(CH₂CH₂O)nCH₂CH₂NH₂, MW = 2000 g mol⁻¹) and Rhodamine B isothiocyanate (RBITC) were purchased from Sigma-Aldrich (USA). Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), 0.25% Trypsin-EDTA, penicillin and streptomycin (100 U/mL), Hank’s Balanced Salt Solution (HBSS) and glutamine were purchased from Life Technologies (France). The pBR322 DNA plasmid (0.5 µg/μL) was purchased from Molecular Biology (France). Tris-EDTA (TE) buffer and Ethidium Bromide (EtBr, 1% solution) were purchased from Thermo Fisher Scientific (USA), Agarose D−5 was purchased from Euromedex (France). All chemical reagents were of analytical grade purity and used without further purification.

Yang X., Salado-Leza D., Porcel E., González-Vargas C.R., Savina F., Dragoe D., Remita H, & Lacombe S. (2020). A Facile One-Pot Synthesis of Versatile PEGylated Platinum Nanoflowers and Their Application in Radiation Therapy. International Journal of Molecular Sciences, 21(5), 1619.

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Synthesis and Characterization of PLGA-based Biomaterials

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Poly(DL-lactide-co-glycolide) 50:50 (PLGA, Mw 45 kDa) was obtained from Boehringer Ingelheim (Belgium); oligo-(l)-lactide (OLL, Mw 5 kDa), oligo-(D)-lactide (OLD, Mw 5 kDa) were synthesized from the respective lactic acid isomers as described earlier [14 (link)]; Dextran sulphate sodium salt (DS) (Mw~9–15 kDa), Poly-l-arginine hydrochloride (PAr, Mw~15–70 kDa), Polyallylamine hydrochloride (PAH, Mw~58 kDa), Poly(sodium 4-styrenesulfonate) (PSS) (Mw~70 kDa), Poly vinyl alcohol (PVA, Mw 70 kDa), Fluorescein isothiocyanate (FITC), Rhodamine B isothiocyanate (RBITC), Resazurin sodium salt, Crystal Violet, EDTA, zimozan were all obtained from Sigma-Aldrich (Saint Louis, MO, USA); Phosphate buffered Saline (PBS), DMEM/F12, trypsin, penicillin and streptomycin were purchased from PanEco (Moscow, Russia); Fetal calf serum (FCS) was obtained from Invitrogen (Carlsbad, CA, USA); Chloroform was obtained from Chimmed (Moscow, Russia); DMSO was obtained from Panreac (Castellar del Vallès, Spain).

Kochetkova O.Y., Demina T.S, & Antonova O.Y. (2022). Universal Microcarriers Based on Natural and Synthetic Polymers for Co-Delivery of Hydrophilic and Hydrophobic Compounds. Polymers, 14(5), 931.

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Alginate-Based Biomaterial Synthesis

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Alginate (MW ≈ 250000 g mol⁻¹) was obtained from FMC Biopolymer. Cellulose filter was purchased from Millipore Inc. 2-(N-morpholino)ethanesulfonic acid (MES), potassium permanganate (KMnO₄), bovine serum albumin (BSA), poly(vinyl alcohol) (PVA, MW = 9000−10000), epigallocatechin gallate (EGCG), fluorescein amine, 1-hydroxybenzotriazole hydrate, rhodamine B isothiocyanate (RBITC), and L-glutamine were purchased from Sigma-Aldrich. PLGA (lactic:glycolic = 50:50, MW = 6000−10000 g mol⁻¹) was purchased from LACTEL. H₂O₂ (30% solution) was purchased from Macron Fine Chemicals. 1-Ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride, Pierce BCA Protein Assay Kit, Pierce Quantitative Peroxide Assay Kit, CellROX Green, propidium iodide, and Penicillin-Streptomycin were purchased from Thermo Scientific.

Seo Y., Leong J., Teo J.Y., Mitchell J.W., Gillette M.U., Han B., Lee J, & Kong H. (2017). Active Antioxidizing Particles for On-Demand Pressure-Driven Molecular Release. ACS applied materials & interfaces, 9(41), 35642-35650.

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Multimodal Nanoformulation for Hypoxia Monitoring

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PFOB, Pluronic F‐127, Iron (III) chloride hexahydrate (FeCl₃ · 6H₂O), LOX from Aerococcus viridans, 2′,7′‐Dichlorofluorescin diacetate (DCFH‐DA), IR‐780 iodide, and rhodamine B isothiocyanate (RBITC) were purchased from Sigma Aldrich (USA). Methylene blue (MB) was purchased from TCI (Japan). Hydroxylamine hydrochloride, sodium lactate (NaL), and tannic acid (TA) were purchased from J&K Scientific (China). ATP and 1,10‐phenanthroline were purchased from Aladdin Reagent (China). Hydrogen peroxide (H₂O₂) assay kit, cell counting KIT‐8 (CCK‐8), ATP assay kit, and lactate assay kit were purchased from Solarbio (China). Live/dead cell imaging kit, image‐iT hypoxia reagent, cellROX deep red reagent, Hoechst 33342, and all cell culture reagents were purchased from ThermoFisher Scientific (USA). PE Annexin V Apoptosis Detection Kit I was purchased from BD Biosciences (USA). All reagents and chemicals were purchased commercially and without further purification.

Tian F., Wang S., Shi K., Zhong X., Gu Y., Fan Y., Zhang Y, & Yang M. (2021). Dual‐Depletion of Intratumoral Lactate and ATP with Radicals Generation for Cascade Metabolic‐Chemodynamic Therapy. Advanced Science, 8(24), 2102595.

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Fluorescent Labeling and Cellular Imaging

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All reagents were used as received from commercial sources. Rhodamine B isothiocyanate (RBITC) and all the pharmacological inhibitions were purchased from Sigma. Ethylenediamine (EDA) and N-(3-Dimethylaminopropyl)-N’-ethylcarb odiimide (EDC) were obtained from Aladdin. 1-Hydroxybenzotriazole (HoBt) was purchased from J & K Chemical. D-(+)-Sucrose was obtained from TCI. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin, streptomycin, Alexa Fluore-488 phalloidin molecular probe, To-PRO-3 iodide molecular probe, and Pro Long-Gold antifade reagent were purchased from Gibco (Life Technologies, Invitrogen, USA). Milli-Q water was used for all experiments.

Liu X., Wu F., Tian Y., Wu M., Zhou Q., Jiang S, & Niu Z. (2016). Size Dependent Cellular Uptake of Rod-like Bionanoparticles with Different Aspect Ratios. Scientific Reports, 6, 24567.

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