HEK293T (human embryonic kidney), HeLa (human cervix adenocarcinoma) and HT29 (human colorectal adenocarcinoma) cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). HEK293T and HeLa cells were cultured in DMEM with 10% FBS (Hyclone, Logan, UT, USA). HT29 cells were maintained with McCoy’s 5a medium (Thermo Fisher Scientific, Waltham, MA, USA) with 10% FBS. Recombinant human TNFα (210-TA) was purchased from R&D systems (Minneapolis, MN, USA). Cycloheximide (C4859) was purchased from Sigma-Aldrich (St. Louis, MO, USA). BV6 and z-VAD-fmk were obtained from Selleckchem (Houston, TX, USA). 5z-7-oxozeaenol used as a TAK1 inhibitor was purchased from Tocris (Bristol, UK).
Recombinant human tnfα 210 ta
Manufactured by R&D Systems
Sourced in United States
Recombinant human TNFα (210-TA) is a laboratory product from R&D Systems. It is a cytokine that plays a role in inflammation and immune regulation.
Automatically generated - may contain errors
Lab products found in correlation
Cycloheximide c4859, by Merck Group (1 mentions)
Mccoy s 5a medium, by Thermo Fisher Scientific (1 mentions)
Z vad fmk, by Selleck Chemicals (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Mouse anti gapdh nb300 221 antibody, by Novus Biologicals (1 mentions)
U0126 9903, by Cell Signaling Technology (1 mentions)
Sb203580 5633, by Cell Signaling Technology (1 mentions)
Hrp conjugated anti rabbit igg 7074, by Cell Signaling Technology (1 mentions)
Nocodazole, by Merck Group (1 mentions)
Dynasore, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
4 protocols using recombinant human tnfα 210 ta
1
Comparative Analysis of Cell Lines
2
Signaling Pathways Activation in TNF-α and CCL21 Response
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Recombinant human TNF‐α (210‐TA) and CCL21 (366‐6C) were purchased from R&D Systems (Minneapolis, MN, USA). The following antibodies were used in Western blot analyses and immunocytochemistry: rabbit anti‐TNF‐α (ab6671), goat anti‐rabbit FITC‐conjugated IgG H&L (ab6717), and rabbit anti‐CCR7 (ab103404) from Abcam (Cambridge, MA, USA); rabbit anti‐p38 (8690), rabbit anti‐phospho‐p38 (Thr180/Tyr182; 4511), rabbit anti‐ERK (9102), rabbit anti‐phospho‐ERK (Thr202/Tyr204; 9101), and HRP‐conjugated anti‐rabbit IgG (7074) antibodies from Cell Signaling Technology (Danvers, MA, USA); and mouse anti‐GAPDH (NB300‐221) antibody from Novus Biologicals (Littleton, CO, USA). The ERK inhibitor U0126 (9903) and the p38 inhibitor SB203580 (5633) were purchased from Cell Signaling Technology.
3
Evaluating the Effects of Nocodazole, Dynasore, and Smac Mimetic on Cellular Signaling
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Nocodazole (M1404: used at 400 ng/ml) and dynasore (D7693: used at 100 μM) were purchased from Sigma (St. Louis, MO, USA). Antibody to VHL (556743) was purchased from BD Biosciences (San Jose, CA, USA). Antibodies to RalA (4799) and RalBP1 (5739) were purchased from Cell Signaling Technology (Beverly, MA, USA). Recombinant human TNF-α (210-TA: used at 17 ng/ml) was purchased from R&D Systems (Minneapolis, MN, USA). Smac mimetic TL32711/birinapant (A-1901: used at 100 nM) (previously known as compound-C49 (link)) was purchased from Active Biochem (Maplewood, NJ, USA). CHX (239763: used at 10 μg/ml) was purchased from Calbiochem (San Diego, CA, USA).
4
Investigating NF-κB Pathway Modulation
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Recombinant human TNFα (210-TA) was acquired from R&D Systems and was reconstituted in sterile PBS containing 0.3% BSA. Diclofenac sodium (DCF, D6899), amiodarone hydrochloride (AMD, A8423), paracetamol (APAP, A7085), and fialuridine (FIAU, SML0632) were obtained from Sigma. Ximelagatran (XIM) was obtained from AstraZeneca. All compounds were dissolved in DMSO (Sigma). The antibodies against pIKKα/β (#2697), IKKβ (#2370), IKKα (#2682), pNFκB (#3031), NFκB (#6956), pIκBα (#2859), and IκBα (#9242) were from purchased from Cell Signaling Technologies. Secondary horseradish peroxidase-coupled antibodies were obtained from Dianova.
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