Irdye 800 conjugated anti mouse antibody

Manufactured by Rockland Immunochemicals

Sourced in United States

The IRDye 800 conjugated anti-mouse antibody is a laboratory reagent designed for use in various immunochemical applications. It is a mouse monoclonal antibody that has been conjugated with the near-infrared fluorescent dye, IRDye 800. This product is intended for research use only and its core function is to detect and quantify mouse target proteins in biological samples.

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Lab products found in correlation

C myc a14, by Santa Cruz Biotechnology (1 mentions) Mouse monoclonal anti α tubulin clone b 5 1 2, by Merck Group (1 mentions) Anti gst z 5, by Santa Cruz Biotechnology (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Mouse monoclonal anti ha, by Fortrea (1 mentions) Anti rad21 antibody, by Abcam (1 mentions)

2 protocols using irdye 800 conjugated anti mouse antibody

Western Blot Antibody Validation

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The primary antibodies used were mouse monoclonal anti-HA (Covance; RRID:AB_2314672), rabbit polyclonal purified antibody c-Myc (A-14) (Santa Cruz Biotechnology, Inc.; RRID:AB_631274) rat monoclonal anti-α-tubulin [YL1/2] (Novus Biologicals; RRID:AB_305328), mouse monoclonal anti-α-tubulin clone B-5-1-2 (Sigma-Aldrich; AB_477579) and rabbit polyclonal anti-GST (Z-5) (Santa Cruz; AB_631586). The secondary antibodies used were IRDye 800 conjugated anti-mouse antibody (Rockland Immunochemicals, Inc; RRID: RRID:AB_10703265), IRDye 800 conjugated anti-rabbit antibody (Rockland Immunochemicals, Inc; RRID:AB_220152), and IRDye700 conjugated anti-rat antibody (Rockland Immunochemicals Inc.; RRID: AB_220171). The blots were analyzed using the Odyssey Infrared Imaging system (LI-COR Biosciences).

Nuñez I., Rodriguez Pino M., Wiley D.J., Das M.E., Chen C., Goshima T., Kume K., Hirata D., Toda T, & Verde F. (2016). Spatial control of translation repression and polarized growth by conserved NDR kinase Orb6 and RNA-binding protein Sts5. eLife, 5, e14216.

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Rad21 Protein Detection and Quantification

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Western blot analysis was performed using a rabbit polyclonal anti-Rad21 antibody (Abcam) at a dilution of 1:1000 as described previously (Xu et al, 2004 (link)). For loading controls, the membranes were subsequently incubated in a mouse monoclonal anti-pan actin antibody (Cell Signalling Technology, Danvers, MA, USA) at a dilution of 1:5000 followed by an IRDye800-conjugated anti-mouse antibody (Rockland, Gilbertsville, PA, USA).
Immunofluorescence detection of RAD21 protein was performed essentially as described previously (Xu et al, 2004 (link)) using a primary rabbit polyclonal antibody (Abcam) (Supplementary Material File 1).

Deb S., Xu H., Tuynman J., George J., Yan Y., Li J., Ward R.L., Mortensen N., Hawkins N.J., McKay M.J., Ramsay R.G, & Fox S.B. (2014). RAD21 cohesin overexpression is a prognostic and predictive marker exacerbating poor prognosis in KRAS mutant colorectal carcinomas. British Journal of Cancer, 110(6), 1606-1613.

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