Akt inhibitor akti

Anti‐BDNF and anti‐TrkB antibodies were bought from Abclonal (Wuhan, China). Anti‐p‐Akt (Ser473), anti‐t‐Akt, anti‐p‐mTOR, anti‐mTOR, anti‐p‐Bad (Ser136), anti‐Bad, anti‐p‐ERK (Thr202/Tyr204), anti‐ERK, anti‐p‐p38 (Thr180/Tyr182), anti‐p38 and anti‐γH2AX (Ser139, 20E3) antibodies were provided by Cell Signaling Technology. Anti‐GAPDH antibody was obtained from Kangchen Biotech Co Ltd (Shanghai, China). Doxorubicin was provided by Aladdin Industrial Company (Shanghai, China, purity 98%). Recombinant human BDNF and TrkB‐Fc were purchased from R&D Systems (Minneapolis, MN, USA). Akt inhibitor (Akti) was provided by Sigma‐Aldrich (St. Louis, MO, USA).

DOX was purchased from Haizheng Pfizer Pharmaceutical Co., Ltd. Levosimendan was obtained from Orion Corporation, Espoo, Finland. The following primary antibodies were purchased from Cell Signaling Technology (Boston, MA, USA): Bcl-2-associated X protein (BAX; 1 : 1000), c-caspase-3 (1 : 1000), PTEN (1 : 1000), P-Akt (1 : 1000), T-Akt (1 : 1000), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1 : 1000), and B-cell lymphoma-2 (Bcl-2) (1 : 1000) was purchased from Abcam. Akt inhibitor (Akt i) was purchased from Sigma-Aldrich (St. Louis, MO, USA). A goat anti-rabbit secondary antibody was purchased from LI-COR Biosciences (Lincoln, USA). The BCA protein assay kit was obtained from Dōjindo Laboratories (Kumamoto, Japan).

Lambda phosphatase was purchased from New England Bio labs (#P0753S). Casein Kinase II Inhibitor I, TBB was purchased from Calbiochem (#218708). CK2α-GST tagged or CK2α untagged recombinant proteins were purchased from Signalchem (#C71-10G-10) and Abcam (#90745) respectively. GST-tagged or full-length BMI1 recombinant proteins were purchased from Mybiosource.com (#MBS717171) and Origene (TP760041) respectively. GST was purchased from Abcam (#89499). Nonradioactive ATP (Adenosine triphosphate) was purchased from Thermofischer (# R1441) and radioactive ATP labeled on the gamma phosphate group with ³²P was purchased from PerkinElmer (#NEG002A250UC). Cyclohexamide, MG132 and AKT inhibitor (Akti) were procured from Sigma (#R750107) and Calbiochem (#474791; 124018) respectively.