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Lsm 6010 scanning electron microscope

Manufactured by JEOL
Sourced in United Kingdom

The JEOL LSM-6010 is a scanning electron microscope (SEM) designed for high-resolution imaging of samples. It features an electron optical column and a specimen chamber that allow for the examination of a wide range of materials at magnifications up to 300,000x. The LSM-6010 is capable of generating detailed images by scanning the surface of a sample with a focused beam of electrons.

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2 protocols using lsm 6010 scanning electron microscope

1

Ependymal Lining Ultrastructure Visualization

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For Scanning Electron Microscopy the ependymal lining of the lateral ventricle was fixed in 2.5% glutaraldehyde, 2% paraformaldehyde in 0.1 m phosphate buffer, incubated in 2% osmium tetroxide, and dehydrated through ethanol solutions. Samples were critical point dried using an Emitech K850 (Quorum Technologies, East Sussex, UK), coated with platinum using a Quorom Q150R S sputter coater (Quorum Technologies). and visualised using a JEOL LSM-6010 scanning electron microscope (Jeol, Herts, UK).
Transmission electron microscopy was performed as previously described.22 (link)
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2

Scanning Electron Microscopy of Ependymal Lining

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For Scanning Electron Microscopy the ependymal lining of the lateral ventricle was fixed in 2.5% glutaraldehyde, 2% paraformaldehyde in 0.1 m phosphate buffer, incubated in 2% osmium tetroxide, and dehydrated through ethanol solutions. Samples were critical point dried using an Emitech K850 (Quorum Technologies, East Sussex, UK), coated with platinum using a Quorom Q150R S sputter coater (Quorum Technologies). and visualised using a JEOL LSM-6010 scanning electron microscope (Jeol, Herts, UK).
Transmission electron microscopy was performed as previously described.22 (link)
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