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Ds fi1 5 megapixel color camera

Manufactured by Nikon
Sourced in Azerbaijan

The DS-Fi1 is a 5-megapixel color camera designed for laboratory applications. It captures digital images with a resolution of 2560 x 1920 pixels.

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5 protocols using ds fi1 5 megapixel color camera

1

Stereoimaging Techniques for Tissue Analysis

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Stereoimages (brightfield and fluorescence) were captured with a Leica MZ10F stereomicroscope and the extended depth-of-focus feature of LAS v3.7 software (Leica Microsystems, Wetzlar, Germany). Brightfield and epifluorescence images of tissue sections were obtained using a Nikon Ti-E inverted microscope equipped with a DS-Fi1 5-megapixel color camera (Nikon Instruments), a CoolSNAP HQ2 14-bit monochrome camera (Photometrics, Tucson, AZ) and NIS Elements software v4.13 (Nikon Instruments).
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2

Bacterial β-Glucuronidase Detection in Roots

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β-Glucuronidase expression by bacteria was detected by staining whole roots in X-Gluc buffer (1 mM 5-bromo-4-chloro-3-indolyl-β-d-glucuronic acid, cyclohexylammonium salt; 0.02% SDS, 50 mM Na-phosphate [pH 7]) (70 (link)) for 48 h. Whole roots were imaged on an AZ100 Multi-Zoom microscope equipped with a DS-Fi1, 5-megapixel color camera (Nikon Instruments, Melville, NY).
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3

Microscopy Imaging of Tissue Sections

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Brightfield and epifluorescence images of tissue sections were obtained using a Nikon Ti-E inverted microscope equipped with a DS-Fi1 5-megapixel color camera (Nikon Instruments), a CoolSNAP HQ2 14-bit monochrome camera (Photometrics, Tucson, AZ) and NIS Elements software v4.13 (Nikon Instruments). Stereoimages (brightfield and fluorescence) were captured with a Leica MZ10F stereomicroscope and the extended depth-of-focus feature of LAS v3.7 software (Leica Microsystems, Wetzlar, Germany).
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4

Cell Culture Imaging Protocol

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Brightfield and fluorescence images of cell cultures were acquired using a Nikon Ti-E inverted microscope attached with a DS-Fi 1 5-megapixel color camera (Nikon Instruments). Images were captured and analyzed using NIS Elements software v4.13 (Nikon Instruments).
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5

Evaluating Purkinje-like Cell Differentiation

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To initially investigate the morphological changes, as well as expression of the CNTN2-mCherry tag introduced by CRISPR-Cas9 into the cells and determine if the cells have successfully differentiated into Purkinje-like cells, fluorescence microscopy was used. Brightfield and fluorescence images were taken using either a Nikon Ti-E inverted microscope attached with a DS-Fi 1 5-megapixel color camera (Nikon Instruments), or an FV3000 inverted confocal microscope (Olympus). NIS Elements software v4.13 (Nikon Instruments) or Fluoview Software (Olympus) were used to capture and analyze the images.
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