Propolis (Henan Bei Yuan Bee products Co., Ltd.), Chitosan (Shandong Aokang Biotechnology Co., Ltd.), Polycaprolactone (Shanghai Yuan Ye Biotechnology Co., Ltd.), Polyvinyl alcohol (Shanghai Aladdin Biochemistry Technology Co., Ltd.), Dichloromethane, N, N-Dimethylformamide and Acetic acid (Yantai Far East Fine Chemical Co., Ltd.), Trypsin- EDTA digestive fluid, Streptomycin mixture and Heparin sodium (Beijing So Lar Bio-Technology Co., Ltd.), 1,1-diphenyl-2-picrylhydrazyl (DPPH) (Shanghai Aladdin Biotech Co.), Commercial membranes (CM): Collagen sponge for hemostasis (Beijing All Gens Medical Instrument Co., Ltd.), Pentobarbital sodium salt (Merck & Co., Inc.), CaCl2 (Tianjin Fengchuan Chemical Reagent Heparin sodium Co., Ltd.), Hematoxylin-eosin, IL-1β, IL-6, TNF-α, Antibodies vimentin, FITC-conjugated goat antirabbit IgG and FITC-conjugated goat anti-mouse IgG were obtained from Service bio, China.
Fitc conjugated goat anti mouse igg
Manufactured by Wuhan Servicebio Technology
Sourced in China
FITC-conjugated goat anti-mouse IgG is a secondary antibody reagent that binds to mouse immunoglobulin G (IgG) and is conjugated to the fluorescent dye fluorescein isothiocyanate (FITC). This allows the detection and visualization of mouse IgG in various immunoassay and immunohistochemistry applications.
Automatically generated - may contain errors
Lab products found in correlation
Hematoxylin eosin, by Wuhan Servicebio Technology (1 mentions)
Tnf α, by Wuhan Servicebio Technology (1 mentions)
Fitc conjugated goat anti rabbit igg, by Wuhan Servicebio Technology (1 mentions)
Pentobarbital sodium salt, by Merck Group (1 mentions)
Chemiluminescence atp determination kit, by Beyotime (1 mentions)
Fv1000, by Olympus (1 mentions)
Ultraview vox spinning disc confocal microscope, by PerkinElmer (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Cy3 conjugated goat anti rabbit igg, by Wuhan Servicebio Technology (1 mentions)
G1202, by Wuhan Servicebio Technology (1 mentions)
Bf8006, by Affinity Biosciences (1 mentions)
Bf9212, by Affinity Biosciences (1 mentions)
G1012, by Wuhan Servicebio Technology (1 mentions)
Fv3000, by Olympus (1 mentions)
5 protocols using fitc conjugated goat anti mouse igg
1
Bioactive Wound Dressing Development
2
CRT expression and ICD markers
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H22, HepG2, or 4T1 cells were treated with PBS, ICG, Cal/ICG, ICG@MPs, Cal@MPs, or Cal/ICG@MPs at the ICG concentration of 4 µg mL−1 and Cal concentration of 60 ng mL−1 for 4 h. The cells were then irradiated with or without 808 nm laser at a power of 1.0 W cm−2 for 5 min. After 4 h incubation, the cells were washed twice with PBS and then incubated with an anti-CRT antibody (R&D Systems, USA, cat. No MAB38981, clone 681233, 2.5 μg mL−1) for 1 h at 4 °C. Subsequently, the cells were further washed twice with PBS and incubated with FITC-conjugated goat anti-mouse IgG (Servicebio, China, cat. No GB22301,1/100 dilution) for 1 h. The expression of CRT was detected using confocal microscopy (FV1000, Olympus) and flow cytometry. The extracellular release of HMGB1 and ATP was examined using the HMGB1 ELISA kit (Moshake, Wuhan, China) and chemiluminescence ATP determination kit (Beyotime, Shanghai, China) according to the manufacturer’s instructions, respectively.
3
Immunofluorescence Analysis of Cadherin Expression
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The cells (5 × 104) were seeded onto glass coverslips in six-well plates and incubated for 24 h. Cells were fixed in 4% paraformaldehyde and blocked with bovine serum albumin. The cells were incubated overnight at 4°C with primary antibodies against E-cadherin (1:200) and N-cadherin (1:100), washed with PBS, and incubated with secondary antibodies (Cy3 conjugated donkey anti-mouse IgG, 1:200, Servicebio, Wuhan, China; FITC-conjugated goat anti-mouse IgG, 1:100, Servicebio, Wuhan, China). DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, St. Louis, MO, USA). Confocal scanning was performed using an Ultraview Vox Spinning Disc confocal microscope (PerkinElmer, MA, USA). The fluorescence intensity of each primary color channel was measured using ImageJ software and the data was illustrated as the mean fluorescence intensity (MFI). A Student's t-test was used to compare the MFIs of different indexes.
4
Immunofluorescence Staining of STING
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After specific treatments, the cells were fixed with pre-warmed 4% formaldehyde buffer at 37 °C for 30 min, washed three times with PBS, then permeabilised with 5% Triton X-100 for 10 min at room temperature washed three times with PBS, then blocked with 1% BSA for 30 min. After incubation with STING (1:50) at 4 °C overnight and FITC conjugated Goat Anti-Mouse IgG (GB22301, ServiceBio, Wuhan, Hubei, China) at 24 ℃ for 1 h, nuclei were stained with DAPI and an anti-fluorescence quencher before mounting on a slide for observation.
5
Immunohistochemical Analysis of Cellular Markers
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The paraffin‐embedded tissue slides were dewaxing and antigen retrieval utilizing citric acid buffer (10 mM, pH 6.0; Servicebio, G1202). Following this, block the glass slides with BSA and incubate overnight at 4°C with the specified primary antibody. The primary antibody employed was as follow: anti‐CCL20 antibody (1:200; Affinity, DF2238); anti‐CD4 antibody (1:500; Elabscience, E‐AB‐22098); anti‐CD116 antibody (1:500; Affinity, DF4820); anti‐alpha SMA (α‐SMA) antibody (1:500; Affinity, BF9212); anti‐vimatin antibody (1:500; Affinity, BF8006). Subsequently, the slides were left at room temperature for 2 h with the appropriate secondary antibodies, which were as follows: Cy3‐conjugated Goat Anti‐Rabbit IgG (1:500; Servicebio, GB21303); FITC‐conjugated Goat Anti‐Mouse IgG (1:200; Servicebio, GB22301). The marked slides were always protected from light and were dyed using DAPI (Servicebio; G1012). The co‐localization of CCL20 with other biomarkers was recorded using confocal laser scanning microscopy (Olympus; FV3000). The fluorescence signal was quantified using ImageJ.
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