Live cell imaging and time-lapse microscopy were performed using a Leica DMI6000B widefield epifluorescence microscope. During imaging, cells were kept at 37 °C in 5% CO2. Images were acquired using a Leica HCX PL APO CS 63 × 1.2 water objective and the Leica LAS AF Software, version 2.6.0.7266. Images were processed with FIJI. Imaging was started at 2 hpi and performed for 48 h (from 2–50 hpi).
Dmi6000b widefield epifluorescence microscope
Manufactured by Leica
The Leica DMI6000B is a widefield epifluorescence microscope. It is designed for fluorescence imaging and analysis applications.
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2 protocols using dmi6000b widefield epifluorescence microscope
1
Live cell imaging of host-pathogen interaction
2
Live Cell Imaging Microscopy Protocol
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Live cell imaging was performed using an inverted Leica DMI6000B wide field epifluorescence microscope with a Leica HCX PL APO CS 63x1.2 water objective, with minimal light intensity and short illumination time. During imaging, cells were kept in 5% CO2 at 37°C. Pictures were taken with the indicated intervals. Confocal images were acquired with an inverted Leica TCS SP8 using a HC PL APO CS2 63×/1.4 NA immersion oil objective. Analysis of fixed cells was done using a Leica DM5500B epifluorescence microscope or a Leica TCS SP8 confocal microscope with a HC PL APO CS2 63×/1.4 NA immersion oil objective. Pearson's correlation coefficient was calculated using the Coloc2 tool of the FIJI software. Image processing was performed using FIJI.
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