Dfc300 fx microscope

Manufactured by Leica

Sourced in Germany

The Leica DFC300 FX is a digital microscope camera designed for high-quality image capture. It features a 3.3 megapixel CCD sensor and supports a wide range of image file formats. The camera can be connected to a computer for image processing and analysis.

Automatically generated - may contain errors

Lab products found in correlation

Transwell chambers 24 well insert 8 μm pore size, by Corning (2 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Transwell plate, by Corning (1 mentions) Horseradish peroxidase hrp labeled anti rabbit igg, by Merck Group (1 mentions)

Close spelling variants of this product

DFC300

5 protocols using dfc300 fx microscope

Cell Migration and Invasion Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

A wound healing assay was used to examine the ability of cell migration. After the cells reached 80% to 90% con uence in the six-well plates, they were wounded by scratching with a 200-μl disposable pipette tip and washed twice with phosphate-buffered saline (PBS). The wounds were photographed at 0, 24, and 48 hours with a DFC300 FX microscope (Leica, Wetzlar, Germany), and their widths were quantitated with ImageJ software (National Institutes of Health, Bethesda, MD, USA).
Transwell migration and invasion assays were performed in 24-well Transwell plates (Corning) inculuding a polycarbonate membrane (8-μm pore size). About 1 × 10 4 cells were plated in the top chamber after transfection. Medium containing 10% PBS was added to the lower chambers. After incubation at 37℃ for 36 hours, the non-invading cells were gently removed with a cotton swab, and the penetrated cells were xed in methanol for 20 minutes and stained with hematoxylin and eosin for 10 and 5 minutes, respectively. Cell numbers were counted under a DFC300 FX microscope (Leica).
Colonial formation 500 MIA PaCa-2 or AsPC-1 cells were plated in 6-well plates. And two weeks later, the cells were xed in methanol for 20 minutes and stained with hexamethylpararosaniline for 10 minutes.

Ding C., Li Y., Wang S., Cheng X., Chen L., Zhang H., Wang Y., & Ding C. (2021). Robo2 Predicts a Better Prognosis and Inhibits Malignant Behavior in Vivo in Pancreatic Ductal Adenocarcinoma.

+ Open protocol

+ Expand

Wound Healing and Migration Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were cultured in 6-well plates at 1 × 10⁶ cells/well in a medium without FBS. When the cells reached 70–80% confluence, they were wounded by scratching with a sterile pipette tip, washed at least three times with phosphate-buffered saline (PBS), and cultured in Opti-MEM^® (Gibco, Life Technologies, Beijing, China). At 0, 12, 24, 36, and 48 h after being wounded, the cells were observed and images were obtained using a DFC300FX microscope (Leica, Jena, Germany).
For transwell migration and invasion assays, we used non-coated or coated membranes in transwell chambers (24-well insert; 8-μm pore size; Corning Life Sciences, Corning, NY, USA). Cells (1 × 10⁴) were plated in the top chamber and cultured with no serum medium after transfection in 6-well plates for 24 h. A medium containing 10% FBS was added to the lower chamber. After incubation at 37°C in 5% CO₂ for 36 h, unpenetrated cells in the upper chamber were wiped with a cotton swab, and the penetrated cells were fixed in methyl alcohol for 20 min and subjected to hematoxylin–eosin (H&E) staining 10 and 5 min, respectively, for counting. Cell numbers were counted under a DFC300FX microscope.

Wang Y., Yuan D., Zhou L., Liang Z., Zhou W., Lu J., Jiang B., You L., Guo J, & Zhao Y.P. (2020). Transducin-Like Enhancer of Split-1 Inhibits Malignant Behaviors in vitro and Predicts a Better Prognosis in Pancreatic Ductal Adenocarcinoma. Frontiers in Oncology, 10, 576.

+ Open protocol

+ Expand

Evaluating Cell Migration and Invasion

Check if the same lab product or an alternative is used in the 5 most similar protocols

In wound-healing assay, the cells were cultured in 6-well plates at 3 × 10⁵ cells/well in FBS-free medium. When cells reached 70–80% confluence, sterile pipette tips were used to scratch and form a “wound”. In transwell migration and invasion assay, we used non-coated and coated membranes in transwell chambers (24-well insert; 8-μm pore size; Corning Life Sciences, Corning, NY, USA) respectively. Preprocessed cells (3 × 10⁴) were added in the upper chamber with FBS-free medium and medium containing 10% FBS was added to the lower chamber. After cultured in cell incubator for 48 h, a cotton swab was used to wipe the unpenetrated cells in the upper chamber, and the penetrated cells were fixed in methyl alcohol for 20 min and then subjected to hematoxylin and eosin staining 10 min and 5 min respectively for counting. The “wound” and the stained cells were observed and photographed via a DFC300FX microscope (Leica, Jena, Germany). The width of “wound” and cell number were further obtained using Image J software (NIH, Bethesda, MD, USA).

Wang Y., Kong Y., Yang Q., Zhong C, & Zhou D. (2024). Identification of fibronectin type III domain containing 3B as a potential prognostic and therapeutic target for pancreatic cancer: a preliminary analysis. European Journal of Medical Research, 29, 221.

+ Open protocol

+ Expand

Microscopic Analysis of Fluorescent Strains

Check if the same lab product or an alternative is used in the 5 most similar protocols

Selected strains were cultivated as described in section “Cultivation conditions.” The cultivation broth was diluted 5-fold and 5 µl of the sample were loaded onto a microscope slide. Microscopy was performed using 100x magnification and immersion oil in a Leica DFC300 FX microscope equipped with a Leica EL600 external light source. Green fluorescence images were obtained using a GFP filter cube, while the red signal was obtained using a Y3 filter cube.

Petkevicius K., Wenning L., Kildegaard K.R., Sinkwitz C., Smedegaard R., Holkenbrink C, & Borodina I. (2022). Biosynthesis of insect sex pheromone precursors via engineered β-oxidation in yeast. FEMS Yeast Research, 22(1), foac041.

+ Open protocol

+ Expand

Ovarian Morphology and C1QTNF3 Distribution

Check if the same lab product or an alternative is used in the 5 most similar protocols

The ovaries were fixed with 4% paraformaldehyde and embedded in the paraffin. After the ovaries were longitudinally and serially sectioned at 5 µm, the sections were placed onto l-lysine treated slides. Serial sections were deparaffinized and hydrated, and then stained with hematoxylin and eosin for analyzing ovarian morphology and the proportion of follicles at different developmental stages.
Immunohistochemical analysis was performed to detect the distribution of C1QTNF3 in the ovary. Briefly, sections were treated as follows: microwave antigen retrieval (700 W for 7 min, twice in 10 mM sodium citrate, pH 6.0); 3% hydrogen peroxide to block endogenous peroxidase and 10% normal goat serum to block non-specific bindings. C1QTNF3 was detected with C1QTNF3 antibody (Ab) (1:400, Abcam, Cat. No. ab36870) and horseradish peroxidase (HRP)-labeled anti-rabbit IgG (1:1000, Sigma-Aldrich, Cat. No. A0545). 3, 3′-diaminobenzidine tetrahydrochloride was used to chromomeric reaction and hematoxylin was used to stain the nucleus. The staining of C1QTNF3 signals was examined by Leica DFC300 FX microscope. For the negative control, the primary Ab was replaced by normal rabbit serum.

Mao Z., Yang L., Lu X., Tan A., Wang Y., Ding F., Xiao L., Qi X, & Yu Y. (2018). C1QTNF3 in the murine ovary and its function in folliculogenesis. Reproduction (Cambridge, England), 155(4).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!