A mouse monoclonal antibody obtained from the supernatant of a hybridoma was used to detect JCPyV VP1 (PAB597). Rabbit polyclonal primary antibodies were used to recognize the 5-HT2Rs and TFR in the proximity ligation assay. Anti-5-HT2AR and anti 5HT2CR were obtained from LS Biosciences, Inc cat# LS-A1106 and LS-A1119 respectively. Anti-5-HT2AR recognizes the N terminus while anti-5-HT2CR the C terminus. Anti-5-HT2BR was obtained from Novus Biologicals cat# NLS1111 and recognizes the second extracellular loop. Anti-TFR was purchased from Novus Biologicals cat# NB100–92243. For Western Blot analysis a rabbit monoclonal anti-β-arrestin½ was purchased from Cell Signaling Technologies (#4674) and a mouse monoclonal anti-GAPDH was purchased from Sigma-Aldrich (G8795). A Mouse monoclonal anti-pan Cadherin antibody was used as cell surface marker (abcam, ab6528) and Rabbit polyclonal anti-6X His tag was used to stain for truncated receptors (abcam, ab9108).
Anti tfr
Manufactured by Novus Biologicals
Anti-TFR is a lab reagent used for the detection and quantification of the transferrin receptor (TFR) protein. It is a common marker used in cellular and molecular biology research.
Automatically generated - may contain errors
2 protocols using anti tfr
1
Antibody Detection of JCPyV VP1 and 5-HT2 Receptors
2
Antibody Detection of JCPyV VP1 and 5-HT2 Receptors
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A mouse monoclonal antibody obtained from the supernatant of a hybridoma was used to detect JCPyV VP1 (PAB597). Rabbit polyclonal primary antibodies were used to recognize the 5-HT2Rs and TFR in the proximity ligation assay. Anti-5-HT2AR and anti 5HT2CR were obtained from LS Biosciences, Inc cat# LS-A1106 and LS-A1119 respectively. Anti-5-HT2AR recognizes the N terminus while anti-5-HT2CR the C terminus. Anti-5-HT2BR was obtained from Novus Biologicals cat# NLS1111 and recognizes the second extracellular loop. Anti-TFR was purchased from Novus Biologicals cat# NB100–92243. For Western Blot analysis a rabbit monoclonal anti-β-arrestin½ was purchased from Cell Signaling Technologies (#4674) and a mouse monoclonal anti-GAPDH was purchased from Sigma-Aldrich (G8795). A Mouse monoclonal anti-pan Cadherin antibody was used as cell surface marker (abcam, ab6528) and Rabbit polyclonal anti-6X His tag was used to stain for truncated receptors (abcam, ab9108).
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