R848, MyD88 knockdown constructs, and noble agar were from Sigma (St Louis, MO, USA). SB203580, PD08959, and SP600125 were obtained from Selleck Chem (Boston MA, USA). MTT reagent was obtained in the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega Madison WI, USA). p-p38, MyD88, α-tubulin, and actin antibodies were obtained from Cell Signaling Technologies (Beverly MA, USA). GAPDH was from Santa Cruz (Carlsbad, CA, USA). CD11b and CD14 antibodies were obtained from EBiosciences (San Diego CA, USA). Anti-Il-8 antibody was obtained from Abcam (Cambridge MA, USA). OCI-AML3 was obtained from DSMZ (Braunschweig, Germany) and 293T, HCT116, HL60, THP-1, and U937 cells were obtained from ATCC (Manassas, VA, USA). Primary AML human bone marrow cells were obtained from the CWRU Hematopoietic Stem Cell Core Facility (Cleveland, OH, USA). The cDNA for TLR8 was cloned into the pLVX-EF1alpha-IRES-mCherry vector obtained from Clontech (Mountain View, CA, USA)
Cd11b and cd14 antibodies
Manufactured by Thermo Fisher Scientific
Sourced in United States
The CD11b and CD14 antibodies are cell surface markers used in flow cytometry analysis. CD11b is expressed on the surface of myeloid cells, including monocytes, macrophages, and granulocytes. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein primarily expressed on the surface of monocytes and macrophages. These antibodies can be used to identify and quantify specific cell populations in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Celltiter 96 aqueous one solution cell proliferation assay, by Promega (2 mentions)
Hct116, by American Type Culture Collection (2 mentions)
Noble agar, by Merck Group (2 mentions)
α tubulin, by Cell Signaling Technology (2 mentions)
Thp 1, by American Type Culture Collection (2 mentions)
Mtt reagent, by Promega (2 mentions)
Hl 60, by American Type Culture Collection (2 mentions)
Oci aml3, by Leibniz Institute DSMZ (2 mentions)
Plvx ef1alpha ires mcherry vector, by Takara Bio (2 mentions)
Myd88, by Cell Signaling Technology (2 mentions)
Anti il 8 antibody, by Abcam (2 mentions)
Myd88 knockdown constructs, by Merck Group (2 mentions)
Pd08959, by Selleck Chemicals (2 mentions)
Sp600125, by Selleck Chemicals (2 mentions)
Sb203580, by Selleck Chemicals (2 mentions)
Gapdh, by Santa Cruz Biotechnology (2 mentions)
2 protocols using cd11b and cd14 antibodies
1
Investigating TLR8 Signaling in AML
2
Investigating TLR8 Signaling in AML
Check if the same lab product or an alternative is used in the 5 most similar protocols
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R848, MyD88 knockdown constructs, and noble agar were from Sigma (St Louis, MO, USA). SB203580, PD08959, and SP600125 were obtained from Selleck Chem (Boston MA, USA). MTT reagent was obtained in the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega Madison WI, USA). p-p38, MyD88, α-tubulin, and actin antibodies were obtained from Cell Signaling Technologies (Beverly MA, USA). GAPDH was from Santa Cruz (Carlsbad, CA, USA). CD11b and CD14 antibodies were obtained from EBiosciences (San Diego CA, USA). Anti-Il-8 antibody was obtained from Abcam (Cambridge MA, USA). OCI-AML3 was obtained from DSMZ (Braunschweig, Germany) and 293T, HCT116, HL60, THP-1, and U937 cells were obtained from ATCC (Manassas, VA, USA). Primary AML human bone marrow cells were obtained from the CWRU Hematopoietic Stem Cell Core Facility (Cleveland, OH, USA). The cDNA for TLR8 was cloned into the pLVX-EF1alpha-IRES-mCherry vector obtained from Clontech (Mountain View, CA, USA)
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