Trp53flox flox mice

Manufactured by Jackson ImmunoResearch

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Trp53flox/flox mice are genetically engineered mice with the p53 gene flanked by loxP sites. This allows for tissue-specific or cell-type-specific deletion of the p53 gene through the use of Cre recombinase.

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Lab products found in correlation

C57bl 6j strain, by CLEA Japan (1 mentions) Kraslsl g12d mice, by Jackson ImmunoResearch (1 mentions) Balb ca nu nu nude mice, by CLEA Japan (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions) Rosa26 lacz mice, by Jackson ImmunoResearch (1 mentions) Dneasy blood and tissue kit, by Qiagen (1 mentions) Pdx1 cre mice, by Jackson ImmunoResearch (1 mentions)

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Trp53flox/flox (6 citations) Trp53flox/+, (2 citations)

4 protocols using trp53flox flox mice

Generation of Genetically Engineered Mice

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Female mice of the C57BL/6 J strain and Balb/cA^nu/nu (nude mice) at 5 weeks of age were purchased from CLEA Japan Inc. (Tokyo, Japan). Conditional knock-in mice heterozygous for the Lox-STOP-Lox-KrasG12D allele (hereafter referred to as Kras^LSL-G12D/+ mice) and Trp53^flox/flox mice were obtained from the Jackson Laboratory (Bar Harbor, ME, USA) and maintained in a C57BL/6 J background. These mice were intercrossed to generate Kras^LSL-G12D/+; Trp53^flox/flox mice. Genotyping was performed after weaning as previously described [28 (link),50 (link),]. Animal studies were carried out with the approval of the Chiba Cancer Center for Ethics in Animal Experimentation.

Maru Y., Tanaka N., Tatsumi Y., Nakamura Y., Itami M, & Hippo Y. (2021). Kras activation in endometrial organoids drives cellular transformation and epithelial-mesenchymal transition. Oncogenesis, 10(6), 46.

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Conditional Trp53 Deletion in Colonic Stem Cells

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To delete exons 2–10 of Trp53 in colonic stem cells, Trp53^flox/flox mice (Jackson, 008462) or Rosa26-LacZ mice (Jackson, 003474) were crossed with Lgr5-^{EGFP-IRES-creERT2} mice (15 (link)) (Jackson, 008875). All mice were crossed onto a C57BL/6 background for at least 10 generations. Details related to the tamoxifen induced Cre-mediated recombination in the colon of Lgr5-^EGFP-CreERT2 / R26R-^LacZ reporter mice have been reported (16 (link)). Mice were maintained on an AIN-76A semi-purified diet (Research Diets, D10001), fed ad libitum and housed on a 12h-12h light-dark cycle. For genotyping analysis, DNA was extracted from tails using DNeasy Blood and Tissue Kit (Qiagen, 69506). PCR was performed using the following primers: p53flox (5'-GGTTAAACCCAGCTTGACCA-3' and 5'-GGAGGCAGAGACAGTTGGAG-3') and cre recombinase (5'-GCATTACCGGTCGATGCAACGAGTG-3' and 5'-GAACGCTAGAGCCTGTTTTGCACGTTC-3').

Davidson L.A., Callaway E., Kim E., Weeks B.R., Fan Y.Y., Allred C.D, & Chapkin R.S. (2015). Targeted deletion of p53 in Lgr5-expressing intestinal stem cells promotes colon tumorigenesis in a preclinical model of colitis-associated cancer. Cancer research, 75(24), 5392-5397.

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Generation of KPC Pancreatic Cancer Model

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Kras^LSL‐G12D/+ mice (008179#), Trp53 ^flox/flox mice (008462#), and Pdx1‐Cre mice (014647#) were purchased from The Jackson Laboratory (ME, USA). Kras^LSL‐G12D/+ and Pdx1‐Cre mice were crossed with Trp53^flox/flox mice. After two generations, Kras^LSL‐G12D/+; Trp53^flox/flox mice and Pdx1‐Cre; Trp53^flox/flox mice were crossed to generate Kras^LSL‐G12D/+; Trp53^flox/flox; Pdx1‐Cre (KPC) mice. To generate the KPC‐1A cell line, the ascites from a 7‐week‐old male KPC mouse was collected by a sterilized injector and centrifuged at 1000 rpm for 5 min. After washing with PBS twice, the remaining cells were cultured with Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), 100 U mL⁻¹ penicillin, and 100 µg mL⁻¹ streptomycin. After subculturing for 3 generations, a stable adherent cell line was obtained and named KPC‐1A.

Gong J., Liu Y., Wang W., He R., Xia Q., Chen L., Zhao C., Gao Y., Shi Y., Bai Y., Liao Y., Zhang Q., Zhu F., Wang M., Li X, & Qin R. (2023). TRIM21‐Promoted FSP1 Plasma Membrane Translocation Confers Ferroptosis Resistance in Human Cancers. Advanced Science, 10(29), 2302318.

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Genetically Engineered Mouse Models

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Kras^ex3op mice were previously described (Pershing et al., 2015 (link)), Trp53^flox/flox mice (Marino et al., 2000 (link)) were purchased from The Jackson Laboratory (Jax # 008462), and Sftpc^CreER/CreER mice (Xu et al., 2012 (link)) were kindly provided by Mark Onaitis (University of California at San Diego). Sftpc^CreER/CreER;Trp53^flox/flox; Kras^ex3op mice were from a mixed 129 × C57BL/6 background. Kras^ex3op mice used for mutagenesis studies were from a pure 129 background. Mice were genotyped using the following primers:
Kras native and ex3op alleles:
Sftpc^CreER allele:
Trp53 allele:
All animal experiments were approved by Duke IACUC.

Li S, & Counter C.M. (2021). Signaling levels mold the RAS mutation tropism of urethane. eLife, 10, e67172.

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