Gaba elisa kit

To measure gamma-aminobutyric acid (GABA) level in astrocyte, GABA concentration was detected using GABA ELISA kit (LS Bio, WA, USA) according to the manufacturer’s instructions. Astrocyte cell lysate was mixed with detection reagent A and incubated for 1 h at 37 ℃. After incubation, the detection reagent B was added, followed by 30 min incubation at 37 ℃. After incubation, TMB substrate solution was added and incubated for 10 min at 37 ℃. Finally, after incubation, stop solution was added and GABA concentration was detected at 450 nm using an Epoch microplate reader.

Mice were deeply anesthetized and fixed in a stereotaxic apparatus. A guide cannula was implanted into the mPFC and then a microdialysis probe was mounted inserted through the guide cannula and mounted to a CMA microinjection pump to continuously perfuse aCSF at a constant flow rate of 1 µl/min. Samples were collected with a microfraction collector. D-serine concentrations were measured by high-performance liquid chromatography (HPLC) as described previously [38 ]. ATP, glutamate, and GABA levels were quantified with the ATP Bioluminescence Assay Kit (Roche, Mannheim, Germany), glutamate colorimetric assay kit (BioVision, Milpitas, CA, USA), and GABA ELISA kit (LSBio, Seattle, WA, USA) according to the manufacturer’s instructions.