Sephadex 200 10 300 gl

Manufactured by GE Healthcare

Sourced in United Kingdom

Sephadex 200 10/300 GL is a size exclusion chromatography media used for the separation and purification of biomolecules such as proteins, peptides, and nucleic acids. It is a dextran-based gel filtration material with a fractionation range suitable for the analysis of medium-sized to large macromolecules.

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Lab products found in correlation

Pd 10 column, by GE Healthcare (2 mentions) N dodecyl β d maltopyranoside ddm, by Anatrace (1 mentions) Triton x 100, by Anatrace (1 mentions) Ni nta agarose, by Qiagen (1 mentions) Sodium meta arsenite, by Merck Group (1 mentions) Luria broth, by Carl Roth (1 mentions) Runblue precast sds page gels, by Abcam (1 mentions) Ni nta bead, by Qiagen (1 mentions) Bio beads, by Bio-Rad (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions) Complete edta free protease inhibitor cocktail tablet, by Roche (1 mentions) Hepes, by Carl Roth (1 mentions)

2 protocols using sephadex 200 10 300 gl

Purification of Membrane Proteins

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The detergents n-Dodecyl-β-D-maltopyranoside (DDM) and Triton X-100 were purchased from Anatrace (USA). The Ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail came from Roche (Switzerland). PD10 columns and the Sephadex 200 10/300 GL were from GE Healthcare Life Sciences (UK) and Ni-NTA Agarose was from Qiagen (Germany). U-¹³C-D-glucose and ¹⁵N-ammonium chloride were purchased from Cortecnet (France) and Cambridge Isotope Laboratories, Inc. (USA). Phosphatidylcholine (EPC) and phosphatidic acid (EPA) from egg yolk came from Sigma Aldrich (USA). E. coli C41(DE3) competent cells were purchased from Lucigen (USA).

Kunert B., Gardiennet C., Lacabanne D., Calles-Garcia D., Falson P., Jault J.M., Meier B.H., Penin F, & Böckmann A. (2014). Efficient and stable reconstitution of the ABC transporter BmrA for solid-state NMR studies. Frontiers in Molecular Biosciences, 1, 5.

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Purification and NMR Analysis of Proteins

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Luria broth, Tris and HEPES were obtained from Carl Roth GmbH & Co. Ni-NTA beads were purchased from Qiagen and Biobeads were ordered from Bio-Rad. ATP and sodium meta-arsenite were purchased from Sigma-Aldrich. Complete EDTA free protease inhibitor cocktail tablets were bought from Roche and Run Blue precast SDS-PAGE gels from Expedeon. Lipids were obtained from Avanti Polar Lipids. All other compounds were from PanReac AppliChem reagents. For size exclusion chromatography Sephadex 200 10/300GL and PD10 column for buffer exchange were from GE Healthcare Life Sciences. Labelled glucose (¹³C) and other amino acids were ordered from Cambridge Isotope Laboratories (CIL) while ammonium chloride (¹⁵N) was obtained from Cortecnet. The Gd³⁺-DOTA complex was bought from BOC sciences.

Kaur H., Lakatos A., Spadaccini R., Vogel R., Hoffmann C., Becker-Baldus J., Ouari O., Tordo P., Mchaourab H, & Glaubitz C. (2015). The ABC Exporter MsbA probed by Solid state NMR – Challenges and Opportunities. Biological chemistry, 396(9-10), 1135-1149.

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