Microscopical analysis was carried out with a Nikon (Tokyo, Japan) Eclipse Ni-U equipped with a DS-Fi2 digital camera, a Nikon Eclipse Ti2 (Tokyo, Japan) and a Leica DMi8 microscope (Wetzlar, Germany).
Eclipse ti2
Manufactured by Leica
Sourced in Japan
The Eclipse Ti2 is a high-performance inverted microscope system designed for advanced cell imaging and analysis applications. It features a stable, ergonomic design and offers a range of advanced optics and imaging capabilities to support a variety of research needs.
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Lab products found in correlation
2 protocols using eclipse ti2
1
Microscopic Analysis with Nikon and Leica
2
Microscopic Analysis of Bacterial Cell Motility
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We characterized the double layer using 2x (Nikon Plan 2x UW, NA 0.06), 2.5x (Leica 2.5x N PLAN, NA 0.07) and 10x (Nikon 10x PLAN FLUOR PH2 DLL, NA 0.3) magnifications in two different microscopes, Nikon Eclipse Ti2 and Leica DMi8. The images were acquired every 2 min in the Nikon Eclipse Ti2 and every 1 min in the Leica Dmi8 using brightfield illumination. Cell motility and cell density were recorded with a 40x objective (Nikon 40x LWD, NA 0.55) at 37°C. Cell motility was measured by adapting a Particle Image Velocimetry (PIV) code written in Matlab (Sveen, 2004 ). The surface coverage was measured by thresholding the images and dividing the area covered by bacteria by the total area of our field of view. The threshold was set by using the command ‘imbinarize’ in Matlab and adapting the sensitivity of its threshold to account for the best estimate of cells in the field of view.
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