Hdac colorimetric assay kit

DEN, 2-AAF, 5,5′-dithiobis-(2-nitrobenzoic acid), thiobarbituric acid, Folin’s reagent, pyrogallol, SOD enzyme, H₂O₂, and bovine albumin were obtained from Sigma Chemical Co. (St. Louis, MO). Primary antibodies of Ki-67, COX-2 (Clone SP 21), iNOS (Ab-1), and NF-kB-P65 (Rel A, Ab-1) were purchased from Thermo Fisher Scientific, Anatomical Pathology, Fremont, USA (1:100 dilutions). GST-p form was obtained from Medical and Biological laboratories Co., Tokyo, Japan (1:1,000 dilution). M30 CytoDeath was purchased from Enzo life Science, USA. Anti-CD68 (ED1), -CD163 (ED-2) (1:300 dilution), and -phosphorylated form of tumor necrosis factor alpha receptor 1 (p-TNFR) (1:200 dilution) antibodies were obtained from Santa Cruz, CA, USA. Safranal (W338907 Aldrich) was obtained from Sigma-Aldrich, USA. CellTiter-Glo luminescent Cell viability assay kit and Caspase-Glo 3/7 luminescent assay kit were obtained from Promega (Woods Hollow. Rd., Madison Wisconsin, USA). HDAC Colorimetric Assay Kit and Human IL-8 ELISA Kit (EZHIL8) were obtained from the Millipore Corporation (28820 Single Oak Drive, Temecula, CA 92590, USA) and the TNF-α ELISA kit came from R&D Systems (Minnesota, USA).

HDAC activity in liver homogenate was measured with HDAC Colorimetric Assay Kit (Millipore Corporation, 28820 Single Oak Drive, Temecula, CA 92590, Catalog number: 17–374).

HDAC Colorimetric Assay Kit (Millipore Corporation, 28,820 Single Oak Drive, Temecula, CA 92590, Catalog number: 17-374) was used to measure HDAC activity in liver homogenate.