Mouse monoclonal proliferating cell nuclear antigen antibody

2-AA (2-aminoanthracene), AF-2 (2-(2-furyl)-3-(5-nitro-2-furyl)-acrylamide), and MeIQ (2-amino-3, 4 dimethylimidazo[4,5-f]quinoline) were obtained from Wako Pure Chemicals (Osaka, Japan). AFB1 (aflatoxin B1), Bovine serum albumin, diethylnitrosamine, ethanol, DMSO, and NaN₃ (sodium azide) of analytical grade were purchased from Sigma-Aldrich (St. Louis, MA, USA). Rabbit polyclonal GST placental form (GST-P) antibody was obtained from MBL (Nagoya, Japan). The mouse monoclonal proliferating cell nuclear antigen (PCNA) antibody was from BioLegend (Santiago, CA, USA). EnVision Doublestain system was obtained from Dako (Glostrup, Denmark). The avidin–biotin–horseradish peroxidase complex (ABC) kit was purchased from Vector Laboratories (Burlingame, CA, USA). ApopTaq peroxidase in situ Apoptosis Detection Kit was obtained from Merck (Kenilworth, NJ, USA). Purezol^TM Isolation Reagent was from Bio-Rad (Hercules, CA, USA). High-Capacity cDNA Reverse Transcription Kit was purchased from Applied Biosystem^TM (Foster City, CA, USA), and SensiFast^TM SYBR Lo-ROX Kit was obtained from Bioline (London, UK). Any other chemicals were of analytical grade and were used without any purification.

Diethylnitrosamine (DEN) and 3,3′-diaminobenzidine tetrahydrochloride hydrate (DAB) were obtained from Sigma Aldrich (St. Louis, MO, USA). Rabbit polyclonal GST-placental form (GST-P) antibody was purchased from MBL (Nagoya, Japan). Mouse monoclonal proliferating cell nuclear antigen (PCNA) antibody was obtained from BioLegend (San Diego, CA, USA). Envision™ G/2 Doublestain System was purchased from Dako (Glostrup, Denmark). VECTASTAIN^® ABC Kit was obtained from Vector Laboratories (Burlingame, CA, USA). ApopTag peroxidase In Situ Apoptosis Detection Kit was acquired from Merck (Kenilworth, NJ, USA). All other chemicals and reagents used in the experiments were of analytical grade.

Bovine serum albumin, diethylnitrosamine, ethanol, and DMSO were of analytical grade and purchased from Sigma-Aldrich (St. Louis, MA, USA). Rabbit polyclonal GST-placental form (GST-P) antibody was obtained from MBL (Nagoya, Japan). Mouse monoclonal proliferating cell nuclear antigen (PCNA) antibody was obtained from BioLegend (Santiago, CA, USA). EnVision Doublestain system was obtained from Dako (Glostrup, Denmark). Avidin-biotin-horseradish peroxidase complex (ABC) kit was obtained from Vector Laboratories (Burlingame, CA, USA). Apoptosis Detection Kit was obtained from Merck (Cambridge, MA, USA)). Any other chemicals were of analytical grade and were used without any purification.