Architect c16000 system

Manufactured by Abbott

Sourced in United States

The Architect c16000 System is a high-throughput, random-access clinical chemistry analyzer designed for use in clinical laboratories. It is capable of performing a variety of clinical chemistry tests, including those for assessing metabolic and organ function. The system is equipped with advanced features to ensure efficient and reliable sample processing.

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Lab products found in correlation

Milliplex map human adipokine magnetic bead panel 2 kit, by Merck Group (1 mentions) Fc500 flow cytometer, by Beckman Coulter (1 mentions) Cytotox 96 non radioactive cytotoxicity assay, by Promega (1 mentions) Trypan blue staining, by Merck Group (1 mentions) Cxp analysis software v2, by Beckman Coulter (1 mentions) Architect i2000sr system, by Abbott (1 mentions)

Spelling variants of this product

Architect c16000 (136 citations) Architect system (97 citations) C16000 (35 citations)

4 protocols using architect c16000 system

Measurement of Adipokines and Inflammatory Markers

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All biomarkers were analyzed using Luminex 200^TM System (Luminex Corporation, Austin, TX, USA). In particular, adiponectin, plasminogen activator inhibitor-1 (PAI-1), and resistin were measured with the Millipore’s MILLIPLEX MAP Human Adipokine Magnetic Bead Panel 1 kit. Interleukin 6 and 8 (IL-6, IL-8), and TNF-α were measured using the Millipore’s MILLIPLEX MAP Human Adipokine Magnetic Bead Panel 2 kit. The intra-assay CVs were all below 10%. Inter-assay CVs were below 15% for adiponectin (range: 0.03–49.9 µg/mL), PAI-I (range: 1.13–78.8 ng/mL), resistin (range: 4.97–83.4 ng/mL), IL-6 (range: 0.01–25.4 pg/mL) and IL-8 (range: 0.24–85.6 pg/mL), and below 20% (range: 0.09–4.00 pg/mL) for TNF-α.
CRP was measured using the MULTIGENT CRP Vario assay (CRPVa) developed and validated for use on the Architect c16000 System (Abbott) for the quantitative immunoturbidimetric determination of this biomarker. Plasma samples were diluted in saline solution to 1:2 dilution factor and antigen-antibody reaction (i.e., agglutination, was detected as an absorbance change (572 nm)). Inter-assay CVs were between 2% (0.45 mg/L values) and 0.26% (45 mg/L values).

Carrión-García C.J., Guerra-Hernández E.J., García-Villanova B., Serafini M., Sánchez M.J., Amiano P, & Molina-Montes E. (2020). Plasma Non-Enzymatic Antioxidant Capacity (NEAC) in Relation to Dietary NEAC, Nutrient Antioxidants and Inflammation-Related Biomarkers. Antioxidants, 9(4), 301.

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Comprehensive Cell Viability Assessment

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Cell viability was assessed by two independent LDH assays; in supernatants measured by ARCHITECT C16000 system (Abbott Laboratories, USA), and measured in phenol-red free supernatants by CytoTox96 Non-Radioactive cytotoxicity assay according to manufacturer’s instructions (Promega, Madison, USA). In the latter, stimulated samples were compared to the non-stimulated samples (viable controls); lysed cells from the same donors served as positive controls. In addition, we employed trypan blue staining (Sigma, St. Louis, USA) and an AnnexinV-PI staining according to the manufacturer’s recommendations (Biovision, Milpitas, USA). In brief, after the indicated stimulations, cells were resuspended in AnnexinV-FITC Staining solution and incubated in the dark for 15 min on ice. PI was added and cells were incubated in the dark for another 5 min on ice. The cells were measured on a FC500 flow cytometer (Beckman Coulter) and the data were analyzed using CXP analysis software v2.2 (Beckman Coulter).

Stoffels M., Zaal R., Kok N., van der Meer J.W., Dinarello C.A, & Simon A. (2015). ATP-Induced IL-1β Specific Secretion: True Under Stringent Conditions. Frontiers in Immunology, 6, 54.

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Serum Biomarkers and Genetic Factors

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Serum was prepared by coagulation at room temperature and after centrifugation the samples were stored at −80°C within one hour after collection. C3 and C3d were measured in serum samples as described [39] (link), [40] (link). All C3 and C3d measurements in this study were performed in a single experiment, except for the final sample in months 14–22. C5a was measured by ELISA at a 1/10 dilution using a commercially available development kit (DuoSet) for human complement component C5a (R&D Systems, Minneapolis, USA). All the samples collected at baseline to month 5 were measured in a single run.
The CFH (Y402H; rs1061170) and ARMS2 (A69S; rs10490924) SNPs were genotyped as described [41] (link). Serum zinc concentration was measured by atomic absorption spectroscopy with the spectrophotometer 1100 B from Perkin Elmer. CRP levels were measured by Abbott Architect C16000 system. The immunoturbidimetric test for CRP was provided by Abbott Diagnostics (Abbott Diagnostics).

Smailhodzic D., van Asten F., Blom A.M., Mohlin F.C., den Hollander A.I., van de Ven J.P., van Huet R.A., Groenewoud J.M., Tian Y., Berendschot T.T., Lechanteur Y.T., Fauser S., de Bruijn C., Daha M.R., van der Wilt G.J., Hoyng C.B, & Klevering B.J. (2014). Zinc Supplementation Inhibits Complement Activation in Age-Related Macular Degeneration. PLoS ONE, 9(11), e112682.

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Anthropometric and Metabolic Profiling

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Anthropometric parameters such as body weight, height, waist circumferences (WC), hip circumferences (HC), and blood pressure (BP) were measured in all subjects according to standard protocols. BMI was defined as the weight per height squared (kg/m²). The waist-hip ratio (WHR) was calculated as the ratio of the waist circumferences and hip circumferences. A fasting blood sample was collected after an overnight fast (>8 hours), and another blood sample was collected after 2 hours as part of the OGTT. Plasma samples were obtained from the blood samples by centrifugation at 4°C and kept at −80°C until use, and all the samples were analyzed within 3 months. Glucose was assayed using the glucose oxidase method. Glycated hemoglobin (HbA1c) was determined using the method of high-performance liquid chromatography (VARIANTTM II and D-10™ Systems, BIO-RAD, USA). Fasting insulin (FINS) was measured using an autoanalyzer (ARCHITECT i2000SR System, Abbott Laboratories, IL, USA). Lipid profiles and liver and kidney functions were detected using a biochemical autoanalyzer (ARCHITECT c16000 System, Abbott Laboratories, IL, USA). The homeostasis model assessment insulin resistance (HOMA-IR) was calculated as follows: HOMA-IR = Fasting insulin level (μU/mL) × Fasting plasma glucose level (mmol/L)/22.5.

Luo Y., Qu H., Wang H., Wei H., Wu J., Duan Y., Liu D, & Deng H. (2016). Plasma Periostin Levels Are Increased in Chinese Subjects with Obesity and Type 2 Diabetes and Are Positively Correlated with Glucose and Lipid Parameters. Mediators of Inflammation, 2016, 6423637.

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