Molt 4

Manufactured by Japanese Collection of Research Bioresources Cell Bank

Sourced in Japan

The Molt-4 is a laboratory equipment used for cell culture and cell-based assays. It provides a controlled environment for the growth and maintenance of Molt-4 cells, a human T-cell leukemia cell line. The Molt-4 equipment ensures the proper temperature, humidity, and gas composition required for the optimal growth and proliferation of these cells.

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Lab products found in correlation

Jurkat, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)

2 protocols using molt 4

3D Culture of Acute Lymphoblastic Leukemia

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The human acute T-lymphoblastic leukemia cell lines CCRF-CEM (CEM) and MOLT4 were purchased from JCRB Cell Bank (Osaka, Japan, CEM:JCRB0033, MOLT-4:JCRB9031) in 2015. The cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum at 37 °C under 5% CO₂ in a humidified atmosphere.
Spheroid (3D) cell culture was performed with Col-Tgel (101 Bio, CA) following manufacture protocol. In brief, 2 × 10⁶ cells/100 μL were seeded in soft medium into 48-well plates. The cells were placed in a standard CO₂ cell culture incubator (37 °C, 5% CO₂).

Oiwa K., Hosono N., Nishi R., Scotto L., O’Connor O.A, & Yamauchi T. (2021). Characterization of newly established Pralatrexate-resistant cell lines and the mechanisms of resistance. BMC Cancer, 21, 879.

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Culture of Leukemia Cell Lines

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Human leukemia cell lines U937, Molt-4, and Jurkat cells were obtained from the Japanese Collection of Research Bioresources (JCRB) Cell Bank. Cells were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) (JRH Biosciences, Corston, UK) at 37.0°C in humidified air with 5% CO₂. Viability of untreated control cells was over 95% in all experiments.

Mitsuhashi Y., Furusawa Y., Aradate T., Zhao Q.L., Moniruzzaman R., Kanamori M., Noguchi K, & Kondo T. (2017). 3-O-trans-p-coumaroyl-alphitolic acid, a triterpenoid from Zizyphus jujuba, leads to apoptotic cell death in human leukemia cells through reactive oxygen species production and activation of the unfolded protein response. PLoS ONE, 12(8), e0183712.

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