Accucore c18 analytical column

Phytochemical analyses were performed using UHPLC-ESI-ORBITRAP-MS/MSwith a Dionex Ultimate 3000RS UHPLC system (Thermo Fisher, Waltham, MA, USA) coupled to a Thermo Q Exactive Orbitrap hybrid mass spectrometer equipped with a Thermo Accucore C18 analytical column (2.1 mm × 100 mm, 2.6 µm particle size). The flow rate was maintained at 0.2 mL/min and the column oven temperature was set to 25 °C ± 1 °C. The mobile phase consisted of methanol (A) and water (B) (both acidified with 0.1% formic acid). The gradient program was as follows: 0–3 min, 95% B; 3–43 min, 0% B; 43–61 min, 0% B; 61–62 min, 95% B; and 62–70 min, 95% B. The injection volume was 2 μL.