The extraction of total cell lysates and western blot were performed according to standard procedure. The antibodies used for immunoblotting were as follows: SULT1A1 (ab124011 and ab191069, Abcam), p53 (sc-126, Santa Cruz Biotechnology), PARP (#95423, Cell Signaling), and γH2AX (#07-164, Millipore). Anti-β-actin monoclonal antibody (Millipore) was used as loading control. The horseradish peroxidase-coupled secondary antibodies (Jackson ImmunoResearch) and SuperSignal™ West Dura Extended Duration Substrate detection system (Thermo Fisher Scientific), and images were visualized using ChemiDoc Imaging System (Bio-Rad).
Supersignal west dura extended duration substrate detection system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The SuperSignal™ West Dura Extended Duration Substrate detection system is a chemiluminescent substrate used for the detection of proteins in Western blotting procedures. It provides extended signal duration for sensitive and quantitative detection of target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin monoclonal antibody, by Merck Group (2 mentions)
Chemidoc imaging system, by Bio-Rad (2 mentions)
Horseradish peroxidase coupled secondary antibody, by Jackson ImmunoResearch (2 mentions)
γh2ax, by Merck Group (1 mentions)
Ab124011, by Abcam (1 mentions)
Ab191069, by Abcam (1 mentions)
P53 sc 126, by Santa Cruz Biotechnology (1 mentions)
Anti p53 sc 126, by Santa Cruz Biotechnology (1 mentions)
2 protocols using supersignal west dura extended duration substrate detection system
1
Cell Lysate Extraction and Western Blot
2
Quantitative Western Blot Analysis
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The total cell lysates extraction and western blot were performed according to standard procedure. The antibodies used were Anti-p53 (sc-126, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and Anti-β-actin monoclonal antibody (Millipore, Burlington, MA, USA) was used as loading control. The horseradish peroxidase-coupled secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA) and SuperSignal™ West Dura Extended Duration Substrate detection system (Thermo Fischer Scientific, Waltham, MA, USA), and images were taken using ChemiDoc Imaging System (Bio-Rad).
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