Bruker AV-300 NMR spectrometer was applied for characterizing 1H NMR spectra. Gel permeation chromatography (GPC) measurements were conducted on a Waters GPC system. The eluant was DMF (containing 0.01 M LiBr) with a flow rate of 1.0 ml/min and monodisperse polystyrene as standard samples. The amount of PTX of drug release experiment and biodistribution experiment were measured by high performance liquid chromatography (HPLC) which was conducted via a PerkinElmer Flexar system. Dynamic light scattering (DLS) which was performed on Malvern Zetasizer instrument (Nano-ZS90) was used to characterize the self-assemble and the sizes of NPs. A JEOL JEM-1011 transmission electron microscopy (TEM, Tokyo, Japan) was used to obtain the morphology images of the self-assemble NPs. Optical microscope (Nikon Eclipse Ti, Optical Apparatus Co., Ardmore, PA, USA) was used to observe histological alterations. The immunofluorescence slides were imaged on a Carl Zeiss LSM 700 confocal laser scanning microscope (CLSM).
Flexar system
Manufactured by PerkinElmer
Sourced in United States
The Flexar system is a versatile and modular high-performance liquid chromatography (HPLC) platform designed for a wide range of analytical applications. It provides reliable and consistent performance for laboratories that require accurate and precise chromatographic separation and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Nano zs90, by Malvern Panalytical (1 mentions)
Confocal laser scanning microscope lsm700, by Zeiss (1 mentions)
Av 300 nmr spectrometer, by Bruker (1 mentions)
Jem 1011 transmission electron microscopy, by JEOL (1 mentions)
Gpc system, by Waters Corporation (1 mentions)
1200 series lc ms system, by Agilent Technologies (1 mentions)
6 protocols using flexar system
1
Comprehensive Characterization of Self-Assembled Nanoparticles
2
Solid-Phase Peptide Synthesis and Purification
3
Primaquine and Carboxyprimaquine Quantification
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Primaquine and carboxyprimaquine were measured by a reversed-phase HPLC system
with a diode array detector (Flexar System - Perkin Elmer Inc., Boston, MA, USA)
after liquid-liquid extraction from the whole blood samples with methyl
tert-butyl ether at pH 3. The separation was carried out in a reversed-phase
column RP-18, 15 cm × 4 mm i.d. (Perkin-Elmer Inc.). The mobile phase consisted
of acetonitrilephosphate buffer, pH 3.5 (30:70) eluate at 1.0 mL/min. Analytes
were recorded at 254 nm. Quinine (1.0 μg/mL) was used as an internal
standard17 . The method was linear in the range from 50 ng/mL to 900 ng/mL. The
limit of detection was 20 ng/mL and the limit of quantification was 30 ng/mL for
both analytes. The mean coefficients of variation within a day and day-to-day
were 12.3% and 16.1%, respectively. The mean recovery of primaquine from whole
blood was 87.3%. For carboxyprimaquine, the mean coefficients of variation
within a day and day-to-day were 14.1% and 17.4%, respectively. The mean
recovery was 88.5%. The stability of primaquine (100 ng/mL) and
carboxyprimaquine in whole blood samples were tested by spiking the analytes in
the biological matrix, which was stored for 120 days at −80 oC. There
were no significant interferences of mefloquine, chloroquine,
desethyl-chloroquine, carboxy-mefloquine, and acetaminophen in the detection of
both analytes.
with a diode array detector (Flexar System - Perkin Elmer Inc., Boston, MA, USA)
after liquid-liquid extraction from the whole blood samples with methyl
tert-butyl ether at pH 3. The separation was carried out in a reversed-phase
column RP-18, 15 cm × 4 mm i.d. (Perkin-Elmer Inc.). The mobile phase consisted
of acetonitrilephosphate buffer, pH 3.5 (30:70) eluate at 1.0 mL/min. Analytes
were recorded at 254 nm. Quinine (1.0 μg/mL) was used as an internal
standard
limit of detection was 20 ng/mL and the limit of quantification was 30 ng/mL for
both analytes. The mean coefficients of variation within a day and day-to-day
were 12.3% and 16.1%, respectively. The mean recovery of primaquine from whole
blood was 87.3%. For carboxyprimaquine, the mean coefficients of variation
within a day and day-to-day were 14.1% and 17.4%, respectively. The mean
recovery was 88.5%. The stability of primaquine (100 ng/mL) and
carboxyprimaquine in whole blood samples were tested by spiking the analytes in
the biological matrix, which was stored for 120 days at −80 oC. There
were no significant interferences of mefloquine, chloroquine,
desethyl-chloroquine, carboxy-mefloquine, and acetaminophen in the detection of
both analytes.
4
Peptide Synthesis and Purification
5
Synthesis and Purification of Peptides
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PEG8NB and lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) were synthesized as described elsewhere [16 (link),49 (link),50 (link)]. Peptides (e.g., CYGGGYC) were synthesized via standard Fmoc coupling chemistry on an automated, microwave-assisted peptide synthesizer (Liberty 1, CEM, Matthews, NC, USA). The crude products were cleaved in a trifluoroacetic acid (TFA) cleavage cocktail composed of 7.6 mL trifluoroacetic acid (TFA), 0.2 mL triisopropylsilane (TIPS), 0.2 mL distilled water, and 400 mg phenol. The cleaved and dried peptides were purified by reverse-phase HPLC (PerkinElmer Flexar system) using 95%/5% (v/v) water/acetonitrile (ACN) with a trace amount (0.1 vol %) of TFA as the starting mobile phase. A linear gradient of ACN was employed to separate the products through a semi-prep peptide C18 column (5 mL/min). The separated products were monitored with a UV/vis detector and the purified peptides were characterized with liquid chromatography coupled with mass spectrometry (1200 series LC/MS system, Santa Clara, CA, USA).
6
HPLC Analysis of Phytochemical Extract
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The freshly prepared extract (200 mg) was reconstituted in 10 mL of 70% v/v ethanol to obtain a stock solution (2% w/v). About 2 mL of the solution was filtered through a 0.45 μm membrane filter into the sample vial.
The HPLC analysis of the extract was carried out on a PerkinElmer Flexar system consisting of a binary pump, Photo Diode Array detector, autosampler, and online degasser. The separation was carried out on the Zorbax 300SB-C18 analytical column (4.6 × 250 mm, 5 μm) from Agilent Technologies with an injection volume of 20 μL and an acquisition wavelength of 280 nm. The mobile phase comprising 0.05% trifluoroacetic acid (A) and methanol (B) was employed with gradient elution at a flow rate of 1 mL/min at ambient temperature. The gradient elution was programmed as follows: 0-3 min, 15% B; 3-23 min, 90% B; 23-26 min, 90% B; 26-27 min, 15% B; and 27-32 min, 15% B [16 (link)].
The HPLC analysis of the extract was carried out on a PerkinElmer Flexar system consisting of a binary pump, Photo Diode Array detector, autosampler, and online degasser. The separation was carried out on the Zorbax 300SB-C18 analytical column (4.6 × 250 mm, 5 μm) from Agilent Technologies with an injection volume of 20 μL and an acquisition wavelength of 280 nm. The mobile phase comprising 0.05% trifluoroacetic acid (A) and methanol (B) was employed with gradient elution at a flow rate of 1 mL/min at ambient temperature. The gradient elution was programmed as follows: 0-3 min, 15% B; 3-23 min, 90% B; 23-26 min, 90% B; 26-27 min, 15% B; and 27-32 min, 15% B [16 (link)].
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