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Fitc conjugated anti pstat3 or mouse igg2a fitc isotype control

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FITC-conjugated anti-pSTAT3 or mouse IgG2a-FITC isotype control are fluorescently-labeled antibodies used for flow cytometry analysis. The FITC-conjugated anti-pSTAT3 antibody specifically binds to the phosphorylated form of STAT3 protein, while the mouse IgG2a-FITC isotype control is a non-specific antibody used as a reference.

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Lab products found in correlation

Lyse fix buffer, by BD (2 mentions) Recombinant human il 6, by BD (2 mentions) Human trustain fcr blocking solution, by BioLegend (2 mentions) Cd16 pe and hla dr apc, by BD (2 mentions) Trustain fcr blocking solution, by BD (2 mentions) Perm buffer 3 solution, by BD (2 mentions)

Spelling variants of this product

FITC Mouse IgG2a (12 citations) FITC-conjugated isotype control (6 citations) Anti-mouse IgG2a (5 citations) IgG2a isotype control (4 citations) FITC-conjugated mouse IgG2a (3 citations) FITC-conjugated IgG2a (3 citations) FITC mouse IgG2a isotype control (2 citations) FITC Mouse Isotype control (2 citations) FITC anti-mouse IgG2a (2 citations) Anti-mouse isotype (2 citations)

2 protocols using fitc conjugated anti pstat3 or mouse igg2a fitc isotype control

1

Evaluating Cytokine-Induced STAT3 Activation

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Blood samples (100 µl) were incubated with recombinant human IL-6 (100 ng/ml; BD Biosciences) at 37°C for 15min. Red blood cells were removed and samples fixed with Lyse/Fix buffer (BD Biosciences). After thorough washing, cells were incubated with Human TruStain FcR Blocking Solution (BioLegend) for 10min followed by incubation with CD16-PE and HLA-DR-APC (BD Biosciences). Cells were permeabilised using pre-chilled Perm buffer III solution (BD Biosciences) and then incubated again with TruStain FcR Blocking Solution, followed by incubation with FITC-conjugated anti-pSTAT3 or mouse IgG2a-FITC isotype control (BD Biosciences). All samples were examined by flow cytometry, and data analysed using the FlowJo software (see above).
Chen M., Lechner J., Zhao J., Toth L., Hogg R., Silvestri G., Kissenpfennig A., Chakravarthy U, & Xu H. (2016). STAT3 Activation in Circulating Monocytes Contributes to Neovascular Age-Related Macular Degeneration. Current Molecular Medicine, 16(4), 412-423.
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2

Evaluating Cytokine-Induced STAT3 Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood samples (100 µl) were incubated with recombinant human IL-6 (100 ng/ml; BD Biosciences) at 37°C for 15min. Red blood cells were removed and samples fixed with Lyse/Fix buffer (BD Biosciences). After thorough washing, cells were incubated with Human TruStain FcR Blocking Solution (BioLegend) for 10min followed by incubation with CD16-PE and HLA-DR-APC (BD Biosciences). Cells were permeabilised using pre-chilled Perm buffer III solution (BD Biosciences) and then incubated again with TruStain FcR Blocking Solution, followed by incubation with FITC-conjugated anti-pSTAT3 or mouse IgG2a-FITC isotype control (BD Biosciences). All samples were examined by flow cytometry, and data analysed using the FlowJo software (see above).
Chen M., Lechner J., Zhao J., Toth L., Hogg R., Silvestri G., Kissenpfennig A., Chakravarthy U, & Xu H. (2016). STAT3 Activation in Circulating Monocytes Contributes to Neovascular Age-Related Macular Degeneration. Current Molecular Medicine, 16(4), 412-423.
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