Antibody (Ab) staining was performed to evaluate the expression of surface markers, transcription factors (T-bet, RORγt and Foxp3) and cytokines (IFNγ and IL-17) in CD4 T cells as described previously (Yang, Weiner, 2009 (link)). Briefly, the cells were first incubated with Abs to the cell-surface markers for 30’ at 4°C, followed by treatment with Cytofix/Cytoperm solution from ebioscience (for T-bet, RORγt and Foxp3) for 1h or BD Bioscience (for IFNγ and IL-17) for 20’–40’. Then cells were stained for transcription factors (T-bet, RORγt and Foxp3) or intracellular cytokines (IFNγ or IL-17) for 30’. Approximately 100,000 live cell events were acquired on a FACSCantoII and analyzed using FlowJo software (Tree Star, Inc.). PE-αIL-17, APC-αIFNγ, Pacific Blue-αT-bet, AF647-αFoxp3, FITC-αCD4, APC-αCD4, Pacific Blue-αCD44 and PE-Cy7-αCD25 were purchased from Biolegend. Fixable Viability Dye eFluor™ 780 and PE-αRORγt were purchased from eBioscience.
Pe αrorγt
Manufactured by Thermo Fisher Scientific
The PE-αRORγt is a laboratory equipment product from Thermo Fisher Scientific. It is an antibody that targets the RORγt (Retinoic acid receptor-related orphan receptor gamma t) protein. The core function of this product is to detect and measure the presence of RORγt in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
αcd4 fitc, by BioLegend (2 mentions)
Fixable viability dye efluor 780, by Thermo Fisher Scientific (2 mentions)
Pe αil 17, by BioLegend (2 mentions)
Apc αifnγ, by BioLegend (2 mentions)
Pacific blue αt bet, by BioLegend (2 mentions)
Af647 αfoxp3, by BioLegend (2 mentions)
Apc αcd4, by BioLegend (2 mentions)
Pacific blue αcd44, by BioLegend (2 mentions)
Pe cy7 αcd25, by BioLegend (2 mentions)
Cytofix cytoperm solution, by Thermo Fisher Scientific (1 mentions)
Facscanto 2, by Tree Star (1 mentions)
2 protocols using pe αrorγt
1
Comprehensive Immune Profiling of CD4 T Cells
2
Multiparameter Flow Cytometry Analysis of T Cell Phenotype and Function
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibody (Ab) staining was performed to evaluate the expression of surface markers, transcription factors (T-bet, RORγt and Foxp3) and cytokines (IFNγ and IL-17) in CD4 T cells as described previously (6) . Briefly, the cells were first incubated with Abs to the cell-surface markers for 30' Approximately 100,000 live cell events were acquired on a FACSCantoII and analyzed using FlowJo software (Tree Star, Inc.). PE-αIL-17, APC-αIFNγ, Pacific Blue-αT-bet, AF647-αFoxp3, FITC-αCD4, APC-αCD4, Pacific Blue-αCD44 and PE-Cy7-αCD25 were purchased from Biolegend. Fixable Viability Dye eFluor™ 780 and PE-αRORγt were purchased from eBioscience.
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