The reagents included in standard assay packages with colorimetric and kinetic methods were obtained from BioMaxima S.A., Lublin, Poland. Thiobarbituric acid, FC (Folin-Ciocalteu) reagent, DPPH, sodium persulfate, toluene, acetone, ethanol, methanol, formic acid, PEG, dichlormethane, diphenylborinic acid aminoethylester (NP) and ethyl acetate were obtained from several companies (Sigma, Fluka, Merck). Standard samples of rutin, kaempferol, and chlorogenic acid were purchased from Sigma, Germany. Ferulic acid, galic acid and quercetin were obtained from Roth, Germany. Neutral formalin solutions were purchased from Chemical Company S.A., Iasi, Romania. All other chemicals and solvents used in the study were of analytical grade.
Dichlormethane
Manufactured by Merck Group
Sourced in Germany
Dichlormethane, also known as methylene chloride, is a colorless, volatile liquid chemical compound. It has the chemical formula CH2Cl2. Dichlormethane is a widely used solvent in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Ethanol, by Merck Group (2 mentions)
Ethyl acetate, by Merck Group (2 mentions)
Citric acid, by Merck Group (2 mentions)
Calcium chloride, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Triethylamine, by Merck Group (2 mentions)
Quercetin, by Carl Roth (1 mentions)
Ferulic acid, by Carl Roth (1 mentions)
Sodium persulfate, by Merck Group (1 mentions)
Chlorogenic acid, by Merck Group (1 mentions)
Kaempferol, by Merck Group (1 mentions)
Rutin, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Toluene, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Thiobarbituric acid, by Biomaxima (1 mentions)
Lactic acid, by Merck Group (1 mentions)
Uric acid, by Merck Group (1 mentions)
5 protocols using dichlormethane
1
Phytochemical Analysis of Bioactive Compounds
2
Artificial Urine Preparation and Characterization
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Silver nitrate (99%), sodium citrate (99%), hydrochloric acid (HCl, 37%), sodium hydroxide (98%), uric acid (99%), ascorbic acid (99%), dopamine hydrochloride (98%), sodium chloride (99.5%), sodium nitrate (99%), citric acid (99.5%), calcium chloride (97%) and tetrahydrofuran (99.9%) were all purchased from Sigma Aldrich. Poly(n-butyl acrylate) COOH, bis(2-oxo-1,3oxazolidin-3-yl)phosphinic chloride (99.5%), triethylamine (99.5%), dichlormethane (99.8%), ethanol (99.5%) used were from Merck. All reagent solutions were prepared in double distilled water. Articial urine samples were prepared according to the recipe provided by Brooks and Keevil. 28 The solution was comprised of 1.1 mM lactic acid (98%), 2.0 mM citric acid (99.5%), 25 mM sodium bicarbonate (99.7%), 170 mM urea (98%), 2.5 mM calcium chloride (97%), 90 mM sodium chloride (99.5%), 2.0 mM magnesium sulphate (99.5%), 10 mM sodium sulphate (99%), 7.0 mM potassium dihydrogen phosphate (99.99%), 7.0 mM dipotassium hydrogen phosphate (99%), and 25 mM ammonium chloride (99.5%) all in Millipore water. The pH of the solution was adjusted to 6.0 via the addition of 1.0 M hydrochloric acid (37%).
3
Synthesis and Characterization of Polymeric Biomaterials
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L-Alanine, Chloroacetylchloride, Sodium Hydroxide, Hydrochloric acid Dimethylformamide, Diethylether, Triethylamine, Ethylacetate, Magnesiumsulfate, Chloroform, Isopropanol, Diethylene glycol, Caprolacton, Stannus(II)-Ethylhexonate, Methacrylic acid anhydride, Methacrylic chloride, Dichlormethane, heptane, hexane, acetone, sodium chloride, phosphate buffered saline were purchased from Sigma Aldrich, Taufkirchen, Germany.
4
GC/MS Analysis of Metabolites
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LiCrosolv Ethanol (gradient grade), Sigma Aldrich (St. Louis, MO, USA) was used for extraction. In GC/MS analysis NO-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) from Sigma-Aldrich (St. Louis, USA) was used as a derivatization reagent while dichlormethane (Gram-mol, Croatia) was used as a solvent for the non-derivatized sample.
5
Covalent Labeling of PLGA Nanoparticles
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Porcine mucin, curcumin, rhodamine B (for covalent labelling of PLGA), dichlormethane, dicyclohexylcarbodiimide, 4-(dimethylamino)pyridine and acetonitrile (ACN) were purchased from Sigma-Aldrich (Steinheim, Germany) and poly(lactic-co-glycolic acid) (PLGA) (Resomer RG 503 H, 50:50 ratio, average Mw = 24,000–38,000 Da) was obtained from Evonik Industries (Darmstadt, Germany). Amphiphilic block copolymer Poloxamer (Pluronic F68, F127, 9400, 6200, 3100, 10500 and 6400) was a kind gift from BASF SE (Ludwigshafen, Germany). Pulmonary human mucus was collected by the endotracheal tube method after informed consent from patients (Winterberg Hospital, Saarbrücken, Germany). AlexaFluor-WGA (wheat germ agglutinin) was purchased from Invitrogen (Oregon, USA). All materials employed in the preparation of nanoparticles were of HPLC grade.
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