Vacuum desk 2

Manufactured by JEOL

Sourced in United States

The Vacuum Desk II is a versatile laboratory equipment designed to provide a controlled vacuum environment. It offers precise vacuum control and monitoring capabilities to support various applications requiring a controlled atmospheric pressure.

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Lab products found in correlation

Jsm 5600, by JEOL (1 mentions) Jsm 6360lv, by JEOL (1 mentions)

2 protocols using vacuum desk 2

CaPP Morphology and Elemental Analysis

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A thin gold layer was deposited on the CaPP surface (BAL-TEC SCD 050; Capovani Brothers Inc., NY, USA) to assess CaPP morphology. Images at ×5000 magnification were obtained on an SEM (JSM-5600, JEOL; Tokyo, Japan) with a 15 kV accelerating voltage, 13 mm Z, and 15 mm WD.
The powder was coated with carbon by vapor deposition (Delton vacuum Desk II, Moorestown, NJ, USA) to characterize the chemical elements of the CaPP particles. Elemental analysis was performed using an EDX detector (Vantage, Acquisition Engine Company, Tokyo, Japan) connected to an SEM (JSM-5600, JEOL, Tokyo, Japan). The EDX system was operated at 15 kV with a collection time of 100 s, 30° incidence, Z = 20 mm, and WD = 20 mm. Three areas of approximately 10 µm² were evaluated.

Guanipa Ortiz M.I., dos Santos J.J., Burga Sánchez J., Rodrigues-Filho U.P., Aguiar F.H., Rischka K, & Lima D.A. (2023). Calcium-Polyphosphate Submicroparticles (CaPP) Improvement Effect of the Experimental Bleaching Gels’ Chemical and Cellular-Viability Properties. Gels, 9(1), 42.

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Scanning Electron Microscopy of C. chinense

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For scanning electron microscopy (SEM), the MS media was discarded, and the C. chinense cells were washed with PBS (to eliminate the MS media) and incubated with FAA solution (40% formaldehyde, 50% ethanol, 5% acetic acid, and 5% distilled water) for 72 h at 25 °C with gentle agitation every 3 h. The samples were washed with PBS to eliminate the FAA solution. The cells were dehydrated in ethanol solutions in a sequential gradient at 30%, 50%, 70%, 96%, and 100% for 12, 12, 3, 2 and 1 h, respectively. After the cells were fixed, the samples were dried to the critical point with liquid CO 2 using a Sandri-795 critical point dryer (Tousimis), metalized with gold (Denton vacuum Desk II) and observed using SEM (JEOL JSM 6360LV).

Sanchez‐Sandoval E., Di-Palma G.E.R., González-Mendoza V.M., Cab-Guillén Y.A., Muñoz-Sanchez A., Ramos-Diaz A.L., & Hernández‐Sotomayor S.T. (2020). Phospholipid signaling pathway in Capsicum chinense suspension cells as a key response to a consortium infection.

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