Fab16991g 100

Manufactured by Miltenyi Biotec

The FAB16991G-100 is a laboratory equipment product from Miltenyi Biotec. It is a core component designed to perform a specific function in a laboratory environment. No further details are provided to maintain an unbiased and factual approach.

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Lab products found in correlation

Facsaria 2 cell sorter, by BD (1 mentions) Ic0041g, by R&D Systems (1 mentions) Alexa fluor 488 conjugated mouse anti human cd11b, by R&D Systems (1 mentions) Cd163 apc, by Miltenyi Biotec (1 mentions)

2 protocols using fab16991g 100

Isolation of M2 Tumor-Associated Macrophages

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FACS was performed to obtain CD11b⁺/CD163⁺ M2 TAMs and the CD11b⁺/CD163⁻ control TAMs from human GBMs. Briefly, cells dissociated from human GBM tumours were incubated with Alexa Fluor 488-conjugated mouse anti-human CD11b (R&D Systems, FAB16991G-100) and mouse anti-human CD163-APC (Miltenyi, 130-100-612) or control IgG (Miltenyi, 130-098-846 and R&D Systems, IC0041G) for 30 min at 4 °C, followed by FACS using a BD FACSAria II cell sorter.

Shi Y., Ping Y.F., Zhou W., He Z.C., Chen C., Bian B.S., Zhang L., Chen L., Lan X., Zhang X.C., Zhou K., Liu Q., Long H., Fu T.W., Zhang X.N., Cao M.F., Huang Z., Fang X., Wang X., Feng H., Yao X.H., Yu S.C., Cui Y.H., Zhang X., Rich J.N., Bao S, & Bian X.W. (2017). Tumour-associated macrophages secrete pleiotrophin to promote PTPRZ1 signalling in glioblastoma stem cells for tumour growth. Nature Communications, 8, 15080.

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Isolation and Identification of Tumor-Associated Macrophages from GBM

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The cells isolated from human GBM tumors were incubated with Alexa Fluor 488-conjugated mouse anti-human CD11b (R&D Systems, Minneapolis, MN; FAB16991G-100) and CD163-APC (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany; 130-100-612) or control immunoglobulin G (IgG) (Miltenyi, 130-098-846 and R&D Systems, IC0041G) for 30 min at 4 °C. FACS was performed to sort TAMs using a BD FACSAria II cell sorter (Becton, Dickinson and Company, NJ).

Zhao G., Ding L., Yu H., Wang W., Wang H., Hu Y., Qin L., Deng G., Xie B., Li G, & Qi L. (2022). M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties. Cell Death Discovery, 8, 350.

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