Horseradish peroxidase hrp conjugated anti mouse and anti rabbit igg secondary antibodies

All ligands, LPS, Pam3CSK4 (PAM), and R848, were purchased from InvivoGen (San Diego, CA, USA). Ubiquitin ligase inhibitor (ULI) small-molecule enhancer of rapamycin (SMER) 3 was purchased from Selleckchem (Houston, TX, USA). Protein A-Sepharose beads were purchased from Sigma (St. Louis, MO, USA). Phospho-specific antibodies against ERK1/2, p38, c-Jun NH(2)-terminal kinase (JNK), TAK1, as well as TRAF6, K63-specific polyubiquitin and β-actin were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against p38 and polyubiquitin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Horseradish peroxidase (HRP)-conjugated anti-mouse and anti-rabbit IgG secondary antibodies were purchased from Cell Signaling Technology, and HRP-conjugated anti-goat antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and FITC-labeled anti-CD11b was purchased from BD Biosciences (San Jose, CA, USA).