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Hpa036985

Manufactured by Merck Group
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HPA036985 is a laboratory instrument designed for general scientific applications. It is a versatile piece of equipment that can be used for a variety of tasks. The core function of this product is to provide a reliable and accurate platform for experimental procedures and data collection in a laboratory setting.

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Lab products found in correlation

Ms 261 po, by Thermo Fisher Scientific (2 mentions) Sab2700438, by Proteintech (2 mentions) Anti oxphos complex 4 subunit 4, by Thermo Fisher Scientific (2 mentions) Anti pdhe1α, by GeneTex (2 mentions) Hrp conjugated secondary antibody, by Vector Laboratories (1 mentions) Ab32988, by Abcam (1 mentions) Chemidoc instrument, by Bio-Rad (1 mentions)

3 protocols using hpa036985

1

Mitochondrial Protein Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
anti-FASTKD2 (Proteintech, 17464-1-AP), anti-GRSF1 (Sigma, HPA036985), anti-FASTKD5 (Sigma, SAB2700438), anti-TFAM (Proteintech, 22386-1-AP), anti-mtSSB (Proteintech, 12212-1-AP), anti-PHB (Thermo Fisher Scientific, MS-261-PO), anti-OxPhos Complex IV subunit IV (clone 20E8C12,Thermo Fisher Scientific, A21348), anti-PDHE1α (GeneTex, GTX104015). All primary antibodies were diluted 1:2000.
Rey T., Zaganelli S., Cuillery E., Vartholomaiou E., Croisier M., Martinou J.C, & Manley S. (2020). Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics. Nature cell biology, 22(10), 1180-1186.
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2

Mitochondrial RNA-Interacting Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were separated by SDS-PAGE and transferred to a nitrocellulose membrane. The blots were probed with primary antibodies to known mitochondrial RNA-interacting proteins; LRPPRC (Abcam, ab21864, 1:1000), POLRMT (Abcam, ab32988, 1:5000), SUV3 (kind gift of Dr. Roman Szczesny, 1:5000) and GRSF1 (Sigma, HPA036985, 1:5000), bound by HRP-conjugated secondary antibodies (Vector Laboratories, 1:10000) and detected by ECL in a ChemiDoc instrument (Biorad).
van Esveld S.L., Cansız-Arda Ş., Hensen F., van der Lee R., Huynen M.A, & Spelbrink J.N. (2019). A Combined Mass Spectrometry and Data Integration Approach to Predict the Mitochondrial Poly(A) RNA Interacting Proteome. Frontiers in Cell and Developmental Biology, 7, 283.
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3

Mitochondrial Protein Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
anti-FASTKD2 (Proteintech, 17464-1-AP), anti-GRSF1 (Sigma, HPA036985), anti-FASTKD5 (Sigma, SAB2700438), anti-TFAM (Proteintech, 22386-1-AP), anti-mtSSB (Proteintech, 12212-1-AP), anti-PHB (Thermo Fisher Scientific, MS-261-PO), anti-OxPhos Complex IV subunit IV (clone 20E8C12,Thermo Fisher Scientific, A21348), anti-PDHE1α (GeneTex, GTX104015). All primary antibodies were diluted 1:2000.
Rey T., Zaganelli S., Cuillery E., Vartholomaiou E., Croisier M., Martinou J.C, & Manley S. (2020). Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics. Nature cell biology, 22(10), 1180-1186.
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