Terminal deoxynucleotidyl transferase dutp nick end labelling tunel

Doxorubicin was purchased from Meilunbio (A0520AS). Melatonin was purchased from Yuanye biotechnology (B21269). Dimethyl sulfoxide (DMSO) and Trizol Reagent were purchased from Invitrogen. Primary antibodies against YAP were purchased from Cell Signaling Technology (14074S). Antibodies against Bax and Bcl‐2 were purchased from Proteintech (50599‐2‐lg and 12789‐1‐AP). Antibodies against β‐actin, as well as goat anti‐rabbit and goat anti‐mouse secondary antibodies were purchased from Zhongshan Company. Cell Counting Kit‐8 (CCK‐8) was provided by Dojindo. Terminal deoxynucleotidyl transferase dUTP nick‐end labelling (TUNEL) was purchased from Roche Diagnostics. The 2′,7′‐dichlorofluorescein diacetate (DCFH‐DA), and mitochondrial membrane potential assay kit with JC‐1, were purchased from Beyotime Institute of Biotechnology. Histostain™‐SP Kits (SPN‐9002) and DAB (ZLI‐9017) were purchased from Zhongshan Company. FITC‐labelled wheat germ agglutinin(WGA) was purchased from Sigma (L4895).

Dimethyl sulfoxide (DMSO), trypan blue, paraformaldehyde, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), ganoderic acid A, ganoderic acid G, lucidenic acid A, and ergosterol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco's Modified Eagle Medium, fetal bovine serum, 4′,6-diamidino-2-phenylindole dye (DAPI), dihydroethidium (DHE), fluorescein phalloidin dye, annexin-V-conjugated fluorescein isothiocyanate (FITC) dye, 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide (JC-1) dye, propidium iodide (PI) dye, bicinchoninic acid assay (BCA) kit, phenylmethylsulphonyl fluoride, CM-H₂DCFDA dye, MitoSOX Red, penicillin/streptomycin, protease inhibitor cocktail, 0.25% (w/v) trypsin containing 1 mM EDTA, and phosphate-buffered saline (PBS) were all purchased from Invitrogen (Carlsbad, CA, USA). Terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) and lactate dehydrogenase (LDH) assay kits were obtained from Roche (Basel, Switzerland). Primary antibodies against Nrf2 and Keap1 were obtained from Abcam (Cambridge, UK). Other primary antibodies and secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).