The optical rotations were detected at 20°C using MCP 5100 digital polarimeter (Anton Paar, Graz, Austria). The UV data were recorded on a Shimadzu UV-2500 spectrophotometer (Shimadzu, Kyoto, Japan). The Chirascan CD spectrometer (Applied Photophysics Ltd., Surrey, UK) was used to acquire ECD spectra. 1D and 2D NMR spectra were recorded on a Bruker AVANCE III 500 M NMR spectrometer (Bruker BioSpin Corporation, Billerica, USA). UPLC-Q-TOF/MS analysis was carried out on a Waters ACQUITY UPLC system (Waters Corporation, Milford, USA) equipped with an AB SCIEX Triple TOF 5600 mass spectrometer with electrospray ionization source (ESI; Framingham, MA, USA). Silica gel (200–300 mesh, Qingdao Marine Chemical Inc., Qingdao, China), RP-C18 silica gel (Merck KGaA, Darmstadt, Germany), and Sephadex LH-20 gel (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) were employed for column chromatography (CC). High-performance liquid chromatographies (HPLCs) were performed on Agilent 1260 series (Agilent Technologies, Santa Clara, USA) with C18 reversed-phase columns (YMC, Kyoto, Japan; 250 × 4.6 mm i.d., 5 μm, for analysis; 250 × 10 mm i.d., 5 μm, for separation).
Mcp 5100
Manufactured by Anton Paar
Sourced in Austria
The MCP 5100 is a high-precision density meter developed by Anton Paar. It measures the density of liquids and gases with high accuracy. The instrument uses the oscillating U-tube principle to determine the sample's density.
Automatically generated - may contain errors
Lab products found in correlation
Tripletof 5600 mass spectrometer, by AB Sciex (1 mentions)
Rp c18 silica gel, by Merck Group (1 mentions)
C18 reversed phase column, by YMC (1 mentions)
Agilent 1260 series, by Agilent Technologies (1 mentions)
Uv 2500 spectrophotometer, by Shimadzu (1 mentions)
Waters acquity uplc system, by Waters Corporation (1 mentions)
Sephadex lh 20 gel, by GE Healthcare (1 mentions)
Chirascan cd spectrometer, by Applied Photophysics (1 mentions)
Avance 3 500 m nmr spectrometer, by Bruker (1 mentions)
2 protocols using mcp 5100
1
Spectroscopic Analysis of Molecular Structure
2
A Simple Polarimetry Setup for Teaching
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Polarizing film was purchased from American Polarizer
(AP42-007T-12X19).
Polarizing films intended for physics experiments in schools (from
Amazon.com, without specifications) gave almost the same results and
may be used. Cuvettes/cells used were of optical glass (path length;
1.0, 2.0, 4.0, and 10.0 cm) and purchased through Amazon.com. Plastic
cuvettes cannot be used as plastic depolarizes plane polarized light.
For the measurement of rotation as a function of wavelength, LEDs
with wavelengths in the range 405–660 nm were used. For all
other experiments, a yellow LED with a peak lambda of 592 nm and a
bandwidth of 20 nm was used. SeeSI for
more information and instructions for making the protractor, plastic
dial, and pieces of polarizing film; for assembling the instrument;
and for peforming measurements.
Solutions were made of fructose,
sucrose, arabinose, limonene,
citrus ski wax remover, and some of the honeys and syrups. The essential
oils were used neat. SeeSI for detailed
information.
Rotation measurements were made at minimum light
transmission (lowest
reading of the voltmeter connected to the detector LED) in a dimly
lit room. Control measurements were regularly performed on a commercial
scientific instrument (Anton Paar MCP 5100, 589 nm) for comparison.
Measurements were made at ambient temperature (20–23 °C),
unless otherwise stated. This is in the same range as the literature
values.
(AP42-007T-12X19).
Polarizing films intended for physics experiments in schools (from
Amazon.com, without specifications) gave almost the same results and
may be used. Cuvettes/cells used were of optical glass (path length;
1.0, 2.0, 4.0, and 10.0 cm) and purchased through Amazon.com. Plastic
cuvettes cannot be used as plastic depolarizes plane polarized light.
For the measurement of rotation as a function of wavelength, LEDs
with wavelengths in the range 405–660 nm were used. For all
other experiments, a yellow LED with a peak lambda of 592 nm and a
bandwidth of 20 nm was used. See
more information and instructions for making the protractor, plastic
dial, and pieces of polarizing film; for assembling the instrument;
and for peforming measurements.
Solutions were made of fructose,
sucrose, arabinose, limonene,
citrus ski wax remover, and some of the honeys and syrups. The essential
oils were used neat. See
information.
Rotation measurements were made at minimum light
transmission (lowest
reading of the voltmeter connected to the detector LED) in a dimly
lit room. Control measurements were regularly performed on a commercial
scientific instrument (Anton Paar MCP 5100, 589 nm) for comparison.
Measurements were made at ambient temperature (20–23 °C),
unless otherwise stated. This is in the same range as the literature
values.
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