Ab1903

Ten cases were utilised to investigate tissue expression patterns of MOBP and HIP1. These included MSA cases (N = 5) and healthy controls (N = 5). Eight-micrometre-thick sections were cut from the cerebellar hemispheric FFPE blocks of patients with MSA and healthy controls and immunostained using a standard avidin-biotin-peroxidase complex method with di-aminobenzidine as the chromogen. The antibodies used in this study were MOBP (Atlas Antibodies HPA035152, 1:200; Bioss Antibodies BS-11184R, 1:100), and HIP1 (Abcam ab181238, 1:100; Novus Biologicals NB300–203, 1:2,000). Heat antigen retrieval pre-treatment was used prior to application of the primary antibodies unless otherwise specified. The samples were mounted and examined using a light microscope. Additionally, three cases per disease control group (PD N = 3, PSP N = 3, and HD N = 3) were used to investigate the disease specificity of expression patterns of MOBP and HIP1. Mid brain was investigated in PD, and the frontal cortex in PSP and HD. FFPE tissue sections were processed and stained as described above for the cerebellar samples. FFPE tissue sections were also stained with mouse anti-α-synuclein (Abcam ab1903, 1:1,000), mouse anti-AT8 (Invitrogen MN1020, 1:600), and mouse anti-IC2 (Chemicon MAB1574, 1:1,000, formic acid pre-treatment) for PD midbrain, and frontal cortex for PSP and HD, respectively.