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Lentiviral short hairpin rna shrna transduction particles

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Lentiviral short hairpin RNA (shRNA) transduction particles are a type of lab equipment used for gene knockdown experiments. They are designed to deliver short hairpin RNA (shRNA) sequences into target cells, leading to the knockdown of specific genes of interest through RNA interference (RNAi) mechanisms. The core function of these particles is to facilitate the stable and efficient introduction of shRNA constructs into cells, enabling researchers to study the effects of gene silencing.

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Mission® short hairpin RNA (shRNA) lentiviral transduction particles Short hairpin shRNA lentiviral particles Lentiviral transduction particles Short hairpin RNA ( ShRNA lentiviral particles Lentiviral particles Lentiviral shRNAs

2 protocols using lentiviral short hairpin rna shrna transduction particles

1

Lentiviral shRNA Targeting FABP5

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Lentiviral short hairpin RNA (shRNA) transduction particles targeting FABP5 and the negative control vector were purchased from Sigma‐Aldrich (St. Louis, MO) and transduced into HCC cell lines according to the manufacturer's instructions. Puromycin (2.5 μg/mL) was used to select stable clones. The shRNA sequences are listed in Table S1.
Ohata T., Yokoo H., Kamiyama T., Fukai M., Aiyama T., Hatanaka Y., Hatanaka K., Wakayama K., Orimo T., Kakisaka T., Kobayashi N., Matsuno Y, & Taketomi A. (2017). Fatty acid‐binding protein 5 function in hepatocellular carcinoma through induction of epithelial–mesenchymal transition. Cancer Medicine, 6(5), 1049-1061.
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2

Lentiviral shRNA Knockdown of PRMT1

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Lentiviral short hairpin RNA (shRNA) transduction particles targeting PRMT1 and a negative control vector were purchased from Sigma-Aldrich (St. Louis, MO) and transduced into the HCC cell lines Bel-7404 and YY-8103 according to the manufacturer's instructions. Puromycin (2.5 μg/mL) was used to select stable clones. The shRNA sequences are shown in Table 1.
Zhang X.P., Jiang Y.B., Zhong C.Q., Ma N., Zhang E.B., Zhang F., Li J.J., Deng Y.Z., Wang K., Xie D, & Cheng S.Q. (2018). PRMT1 Promoted HCC Growth and Metastasis In Vitro and In Vivo via Activating the STAT3 Signalling Pathway. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 47(4).
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