Glucose tolerance tests (GTTs) were performed on male and female Mkp-2+/+ and Mkp-2−/− mice fed either chow or HFD for 16 to 24 weeks. Mice were fasted overnight for 16 h followed by intraperitoneally (i.p.) injection of glucose (2 g/kg). Blood glucose levels were measured at time points 0, 15, 30, 60, 90, and 120 min. For insulin tolerance tests (ITTs), mice were fasted for 5 h and were injected (i.p.) with 0.75 mU/g human insulin (Humlin R; Elly and Company, Indianapolis, IN, USA). The blood glucose levels were measured at time points 0, 15, 30, 60, 90, and 120 minutes. Plasma insulin levels were measured using ultra-sensitivity mouse insulin Elisa kit (Crystal Chem, Elk Grove Village, IL, USA; Catalog #90080).
Conscious male chow-fed Mkp-2+/+ and Mkp-2−/− mice aged between 8 to 12 weeks old were individually housed under controlled temperature (23 °C) and lighting (12 h light, 12 h dark cycle, lights on at 0700 h) with free access to food and water. Mice were adapted for one week and kept in metabolic cages (Promethion, Sable Systems, Las Vegas, NV, USA) for one week, and the food and water intake, energy expenditure, respiratory exchange ratio (RER), and physical activity were measured. Data were analyzed using regression analysis using ANCOVA.
Conscious male chow-fed Mkp-2+/+ and Mkp-2−/− mice aged between 8 to 12 weeks old were individually housed under controlled temperature (23 °C) and lighting (12 h light, 12 h dark cycle, lights on at 0700 h) with free access to food and water. Mice were adapted for one week and kept in metabolic cages (Promethion, Sable Systems, Las Vegas, NV, USA) for one week, and the food and water intake, energy expenditure, respiratory exchange ratio (RER), and physical activity were measured. Data were analyzed using regression analysis using ANCOVA.