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Aggrewell system

Manufactured by STEMCELL
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The Aggrewell system is a specialized laboratory equipment designed for the formation of uniform, 3D cellular aggregates. It provides a controlled, high-throughput environment for the generation of spherical cellular structures, which are essential for various applications in cell biology, stem cell research, and tissue engineering.

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Lab products found in correlation

Synthemax, by Corning (1 mentions) Matrigel, by Corning (1 mentions)

Close spelling variants of this product

Aggrewell 400 system AggreWell™800 system AggreWell

2 protocols using aggrewell system

1

Embryoid Body Formation and Cardiomyocyte Differentiation

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Embryoid body (EB) formation was performed using the aggrewell system (StemCell Technologies), harvesting the cells using TrypLE with 1-h ROCKi pre-treatment and seeding an average of 2,000 cells per EB and with ROCKi addition. The EBs were then kept in GMEM 20% FBS and suspension for 2 weeks and then plated onto Matrigel (Corning) or Synthemax (vitronectin, Corning) for 2 more weeks. Beating cardiomyocytes areas were filmed after 3–4 weeks of growth using a light-phase microscope coupled to a NikonE990 camera.
Pijuan-Galitó S., Tamm C., Schuster J., Sobol M., Forsberg L., Merry C.L, & Annerén C. (2016). Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture. Nature Communications, 7, 12170.
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2

Robust Pluripotent Cell Differentiation

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Embryoid body (EB)-based spontaneous differentiation of mESCs and iPSCs at passage 4 was performed using the Aggrewell system (STEMCELL Technologies) according to manufacturer’s instructions. Beating activity was monitored daily from days 8–25 of differentiation across triplicate experiments for each cell line. All iPSC experiments were blinded and the identity of each cell line was only revealed at day 25. Additionally, iPSCs and mESCs were differentiated in several rounds with each iPSC round containing cell lines from each somatic origin to avoid artificial batch effects. L-MEF-iPSC lines 1 and 6 and each mESC line were differentiated in additional triplicate experiments from independent cryopreserved samples to validate consistency of the differentiation protocol.
Hartjes K.A., Li X., Martinez-Fernandez A., Roemmich A.J., Larsen B.T., Terzic A, & Nelson T.J. (2014). Selection via pluripotency-related transcriptional screen minimizes the influence of somatic origin on iPSC differentiation propensity. Stem cells (Dayton, Ohio), 32(9), 2350-2359.
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