Tms 001 c

Manufactured by Merck Group

Sourced in United States

The TMS-001-C is a laboratory equipment product manufactured by Merck Group. It is a core component used for various scientific applications. The device's primary function is to provide precise temperature measurement and control. Further details on the intended use of this product are not available.

Automatically generated - may contain errors

Lab products found in correlation

Tms 002 c, by Merck Group (10 mentions) Es 007 e, by Merck Group (6 mentions) Tms ab2 c, by Merck Group (4 mentions) A5955, by Merck Group (3 mentions) Lm r1637, by Biosera (3 mentions) Fb 1001h, by Biosera (3 mentions) Tms 005 c, by Merck Group (3 mentions) Esg1107, by Merck Group (3 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Es 009 b, by Merck Group (2 mentions) Freezone 2.5 liter freeze dry system, by Labconco (2 mentions) Es 009 c, by Merck Group (2 mentions) Slm 220 b, by Merck Group (2 mentions) Lif2010, by Merck Group (2 mentions) Crl 2836, by American Type Culture Collection (1 mentions) Sml2234, by Merck Group (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Es 008 d, by Merck Group (1 mentions) Knockout dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions) Cellbanker 1 plus, by Takara Bio (1 mentions)

Spelling variants of this product

TMS-001 (2 citations)

13 protocols using tms 001 c

Breast Cancer and Fibrosarcoma Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

The 4T1 (ATCC CRL-2539) and E0771 (94A001,
CH3 BioSystems) breast adenocarcinoma cell lines were maintained in
Dulbecco’s modified Eagle medium (DMEM, LM-D1109, Biosera)
and Roswell Park Memorial Institute medium (RPMI-1640, LM-R1637, Biosera),
respectively, and supplemented with 10% fetal bovine serum (FBS, FB-1001H,
Biosera) and 1% antibiotics (A5955, Sigma). Cell lines were preserved
in 5% CO₂ at 37 °C. MCA205 fibrosarcoma cells (SCC173,
Millipore) were cultured in RPMI-1640 (LM-R1637, Biosera) containing
2 mM l-glutamine (TMS-002-C, Sigma-Aldrich), 1 mM sodium
pyruvate (TMS-005-C, Sigma-Aldrich), 10% FBS (FB-1001H, Biosera),
1× nonessential amino acids (TMS-001-C, Sigma-Aldrich), 1% antibiotics
(A5955, Sigma), and 1× β-mercaptoethanol (ES-007-E, Sigma).

Mpekris F., Papaphilippou P.C., Panagi M., Voutouri C., Michael C., Charalambous A., Marinov Dinev M., Katsioloudi A., Prokopi-Demetriades M., Anayiotos A., Cabral H., Krasia-Christoforou T, & Stylianopoulos T. (2023). Pirfenidone-Loaded Polymeric Micelles as an Effective Mechanotherapeutic to Potentiate Immunotherapy in Mouse Tumor Models. ACS Nano, 17(24), 24654-24667.

+ Open protocol

+ Expand

Murine Breast Cancer and Fibrosarcoma Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

4T1 (ATCC CRL-2539) and E0771 (94A001, CH3 BioSystems) mouse breast adenocarcinoma cell lines were purchased form ATCC and CH3 BioSystems, respectively. The cells were maintained at 37 °C/ 5% CO₂ in Roswell Park Memorial Institute medium (RPMI-1640, LM-R1637, biosera) supplemented with 10% fetal bovine serum (FBS, FB-1001H, biosera) and 1% antibiotics (A5955, Sigma). The MCA205, mouse fibrosarcoma cell line was purchased from Millipore (SCC173, Millipore) and cultured in expansion medium consisting of RPMI-1640 (LM-R1637, biosera) supplemented with 2 mM L-glutamine (TMS-002-C, Sigma), 1 mM sodium pyruvate (TMS-005-C, Sigma), 10 % fetal bovine serum (FBS, FB-1001H, biosera), 1x non-essential amino acids (TMS-001-C, Sigma), 1% antibiotics (A5955, Sigma) and 1x β-mercaptoethanol (ES-007-E, Sigma). MCA205 cells were also maintained at 37 °C/ 5 % CO₂.

Panagi M., Mpekris F., Chen P., Voutouri C., Nakagawa Y., Martin J.D., Hiroi T., Hashimoto H., Demetriou P., Pierides C., Samuel R., Stylianou A., Michael C., Fukushima S., Georgiou P., Papageorgis P., Papaphilippou P.C., Koumas L., Costeas P., Ishii G., Kojima M., Kataoka K., Cabral H, & Stylianopoulos T. (2022). Polymeric micelles effectively reprogram the tumor microenvironment to potentiate nano-immunotherapy in mouse breast cancer models. Nature Communications, 13, 7165.

+ Open protocol

+ Expand

Culturing Fibrosarcoma and Osteosarcoma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

MCA205 fibrosarcoma cells (SCC173, Millipore) were cultured in RPMI-1640 (LM-R1637, biosera) containing 2 mM L-glutamine (TMS-002-C, Sigma-Aldrich), 1 mM sodium pyruvate (TMS-005-C, Sigma-Aldrich), 10 % fetal bovine serum (FBS, FB-1001H, biosera), 1x non-essential amino acids (TMS-001-C, Sigma-Aldrich), 1 % antibiotics (A5955, Sigma) and 1x β-mercaptoethanol (ES-007-E, Sigma). K7M2-WT osteosarcoma cells (CRL2836, ATCC) were cultured in DMEM (LM-S2041, biosera) containing with 10 % FBS and 1 % antibiotics. Cells were incubated at 37 ºC/ 5 % CO₂.

Mpekris F., Panagi M., Charalambous A., Voutouri C., Michael C., Papoui A, & Stylianopoulos T. (2024). A synergistic approach for modulating the tumor microenvironment to enhance nano-immunotherapy in sarcomas. Neoplasia (New York, N.Y.), 51, 100990.

+ Open protocol

+ Expand

Ferroptosis Induction in MCA205 Murine Fibrosarcoma

Check if the same lab product or an alternative is used in the 5 most similar protocols

Murine fibrosarcoma MCA205 cells, which are used in this study, are often used for the analysis of the immunogenicity of dead cancer cells and the development of targeted cancer immunotherapies [2] . MCA205 cells were cultured in RPMI 1640 with L-glutamine (LONZA, BE12-702F) supplemented with 10% FBS (FisherScientific, 11591821), 1% Penicillin/streptomycin (LONZA, DE17-602E), 1% Non-Essential Amino Acids (Sigma-Aldrich, TMS-001-C) and 1% Sodium-Pyruvate. Induction of ferroptosis in MCA205 cells is executed by adding 2.5µM Ras-selective lethal 3 (RSL3, Sigma-Aldrich, SML2234).

Van der Meeren L., Efimova I., Demuynck R., Parakhonskiy B., Krysko D.V, & Skirtach A.G. (2023). Mechanobiology of Ferroptotic Cancer Cells as a Novel "Eat-Me" Signal: Regulating Efferocytosis through Layer-by-Layer Coating. Advanced healthcare materials, 12(28).

+ Open protocol

+ Expand

Derivation and Maintenance of Mouse ESCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse ESCs were cultured on mitomycin-C-treated mouse embryonic fibroblasts (MEFs) in six-well tissue culture plates (92006, TPP, Trasadingen, Switzerland). Mouse ESC maintenance medium was KnockOut™ Dulbecco’s modified Eagle medium [10829018, Thermo Fisher Scientific (Thermo)] supplemented with 20% HyClone™ fetal bovine plasma (SH30070.03, Cytiva), 1% 2-mercaptoethanol (ES-007-E, Merck), 1% nonessential amino acids (TMS-001-C, Merck), 1% nucleosides (ES-008-D, Merck), 1% L-glutamine (TMS-002-C, Merck), 100 U/mL penicillin-streptomycin (15140-122, Thermo), and 1,000 U/mL mouse leukemia inhibitory factor (ESG1107, Merck). Before use, 1 μM mirdametinib [PD0325901/162-25291, FUJIFILM Wako (Wako)] and 3 μM laduviglusib (CHIR-99021/TB4423-GMP, Bio-Techne) were added to mESC maintenance medium. Cells were passaged every 2 to 3 d.
For cryopreservation, CELLBANKER 1plus (CB023, Takara) was used. For thawing, 1 mL of phosphate-buffered saline (PBS, Wako, 048-29805) was added to a cryopreserved cell vial. Mouse ESCs were quickly dispersed by pipetting and transferred to 15-mL centrifuge tubes containing 9 mL of PBS. After centrifugation, the cells were suspended in mESC maintenance medium containing 1 μM PD0325901 and 3 μM CHIR-99021. The cell suspension was then seeded onto MEF feeder cells. The thawed mESCs were used for injection after more than one passage.

Kano M., Mizuno N., Sato H., Kimura T., Hirochika R., Iwasaki Y., Inoshita N., Nagano H., Kasai M., Yamamoto H., Yamaguchi T., Suga H., Masaki H., Mizutani E, & Nakauchi H. (2023). Functional calcium-responsive parathyroid glands generated using single-step blastocyst complementation. Proceedings of the National Academy of Sciences of the United States of America, 120(28), e2216564120.

+ Open protocol

+ Expand

Culturing Mouse Embryonic Fibroblasts and Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse embryonic fibroblasts (MEFs) were derived from 13.5‐dpc embryos. MEFs were maintained in Dulbecco's modified eagle medium (DMEM) (Sigma D5671) medium supplemented with 10% (vol/vol) foetal bovine serum (FBS) (Gibco 10270‐106) and 1 mM L‐glutamine (Merck Millipore TMS‐002‐C). Embryonic stem cells (ESCs) and iPSCs were cultured on mitomycin C (Sigma M4287) treated MEFs in Embryonic stem medium (ESM) containing DMEM (Sigma D5671) supplemented with 15% (v/v) FBS (Gibco 16000‐44), 1 mM L‐glutamine (Merck Millipore TMS‐002‐C), 0.1 mM mercaptoethanol (Merck Millipore ES‐007‐E), 1% nonessential amino acid (NEAA) stock (Merck Millipore TMS‐001‐C), and 1000 U/ml leukaemia inhibitory factor (LIF) (Merck Millipore ESGRO 1107).

Wu L., He S., Ye W., Shen J., Zhao K., Zhang Y., Zhang R., Wei J., Cao S., Chen K., Le R., Xi C., Kou X., Zhao Y., Wang H., Kang L, & Gao S. (2021). Surf4 facilitates reprogramming by activating the cellular response to endoplasmic reticulum stress. Cell Proliferation, 54(11), e13133.

+ Open protocol

+ Expand

Culturing Mouse and Drosophila Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Low-passage E14 mouse embryonic stem cells (mESCs) were grown in
knockout DMEM (Invitrogen, 10829-018) with 15% ESC grade FBS (EMD
Millipore, ES-009-B), 1× L-glutamine (EMD Millipore, TMS-002-C),
1× ES-grade Penicillin/streptomycin (EMD Millipore, TMS-AB2-C),
1× non-essential amino acid (EMD Millipore, TMS-001-C), 0.1%
2-mercaptoethanol, 1× Leukemia Inhibitory Factor (LIF, EMD
Millipore, ESG1107) in 37 °C, 5% CO₂ incubator.
One hour prior to harvest, ∼60-70% confluency cells were
incubated with fresh mESC media. Mouse C3H10T1/2 cells were grown in the
growth media (10% FBS (Hyclone, SH30071.03), Penn/ Strep (Gibco,
15140-122), DMEM (Gibco, 11965-118)) in 37 °C, 5%
CO₂ incubator. Drosophila Schneider 2 (S2)
cells were grown in growth media containing 10% heat inactivated FBS
(Hyclone, SH30071.03), Penn/Strep (Gibco, 15140-122), and
Schneider's Drosophila medium (Gibco, 21720024)) at
room temperature in ambient CO₂.

Shi Z., Fujii K., Kovary K.M., Genuth N.R., Röst H.L., Teruel M.N, & Barna M. (2017). Heterogeneous ribosomes preferentially translate distinct subpools of mRNAs genome-wide. Molecular cell, 67(1), 71-83.e7.

+ Open protocol

+ Expand

Culturing Yeast, Caulobacter, and Mouse ESCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Strains used in this paper are listed at the Experimental Models:
Organisms/Strains in the KEY RESOURCES
TABLE. Yeast strains were grown in YPAD medium (10 g/L yeast
extract, 20 g/L peptone, 40 mg/L adenine sulfate, and 20 g/L glucose) or
Synthetic Dextrose (SD) Medium (6.7 g/L yeast nitrogen base and 20 g/L
glucose plus appropriate amino acids drop out mix). All yeast strains were
cultured at 30 °C, unless specified. Samples were harvested at log
phase (OD₆₀₀ = ∼0.8). Caulobacter
crescentus: NA1000 and its derivatives were grown in
PYE rich medium (1 g/L yeast extract, 2 g/L Bacto peptone, 1 mM
MgSO₄, 0.5 mM CaCl₂) at 28°C. Mouse
embryonic stem cells (ESCs): E14 ESCs (male) (Hooper et al., 1987 ) were cultured in knockout
DMEM (Invitrogen, 10829-018) with 15 % ESC grade FBS (EMD Millipore,
ES-009-B), 1× L-glutamine (EMD Millipore, TMS-002-C), 1×
ES-grade Penicillin/streptomycin (EMD Millipore, TMS-AB2-C), 1×
non-essential amino acid (EMD Millipore, TMS-001-C), 0.1 %
2-mercaptoethanol, 1× Leukemia Inhibitory Factor (LIF, EMD Millipore,
ESG1107) in a 37 °C, 5 % CO₂ incubator.

Fujii K., Susanto T.T., Saurabh S, & Barna M. (2018). Decoding the Function of Expansion Segments in Ribosomes. Molecular cell, 72(6), 1013-1020.e6.

+ Open protocol

+ Expand

Maintenance of DC2.4 Mouse Dendritic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The DC2.4 mouse dendritic cell line was purchased from Millipore (# SCC142). These cells were routinely maintained at 37°C in 5% CO₂ in RPMI supplemented with 10 % heat-inactivated fetal calf serum, 1 X L-Glutamine (TMS-002-C, EMD Millipore, MA, United States), 1X non-essential amino acids (TMS-001-C; EMD Millipore, MA, United States), 1X HEPES Buffer Solution (TMS-003-C; EMD, Millipore, MA, United States), 0.0054X β-Mercaptoethanol (ES-007-E; EMD Millipore, MA, United States), 100 IU/ml Penicillin and 100 μg/ml streptomycin.

Chivero E.T., Liao K., Niu F., Tripathi A., Tian C., Buch S, & Hu G. (2020). Engineered Extracellular Vesicles Loaded With miR-124 Attenuate Cocaine-Mediated Activation of Microglia. Frontiers in Cell and Developmental Biology, 8, 573.

+ Open protocol

+ Expand

Cryopreservation and Lyophilization of Murine ESCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 1

Murine embryonic stem cells (ESC) were maintained feeder free with media containing 205 ml DMEM with glucose/sodium bicarbonate (Millipore, SLM-220-B), 37.5 ml ES Cell Qualified Feeder Bovine Serum (Millipore ES-009-C), 2.5 ml 100× Non-Essential Amino Acids (NEAA) (Millipore TMS-001-C), 2.5 ml L-glutamine solution (Millipore TMS-002-C), 2.5 ml 100× penicillin/streptomycin (Millipore TMS-AB2-C), and 12.5 μl Leukemia Inhibitor Factor (LIF) 10 UG (Millipore, LIF2010).

Approximately 1.0×10⁶ESC were suspended in 0.5 ml phosphate buffered saline with 0.1M trehalose (5) (Sigma Aldrich cat T0167) and 0.3 mg/dl EGCC (Zhejiant Yixin Pharmaceutical, Lanxi, Jinhua, Zhejiang, China) and cryopreserved at −80° C. Lyophilization was done at −80° C. and <0.008 torr using a Labconco Freezone 2.5 liter freeze dry system (Labconco Corporation, Kansas City, Mo.). Lyophilized ESC were stored at room temperature in a trehalose sugar matrix (FIG. 1).

Prior to use for injection, the sugar matrix dissolves when exposed to normal saline, leaving intact but non-viable ESC.

US10813955B2. Methods for treating age-related organ or tissue dysfunction through heterochronic transbiosis using nonviable pluripotent stem cells (2020-10-27). None [US]. Inventors: Richard K. Burt [US].

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!