Pfqnm lc cal probe

MRC-5 cells were cultured at WillCo-dish glass bottom dishes (Willco Wells BV, GWST-5040). Cell images were captured in QI mode under an AFM (JPK NanoWizard 4, Bruker Nano GmbH, Germany). A PFQNM-LC-CAL probe (Bruker Nano GmbH) with an end radius of 75 nm and a force constant of 0.09 N/m was used to acquire topographical images. After an entire cell was imaged by AFM in PBS, the colloid probe (MLCT-O-A probe, Bruker Nano GmbH) with a 20-μm diameter silica sphere was localized on an intact single cell. Young’s modulus was measured using the AFM indentation test based on a Hertz model.

MRC-5 cells (1.0 × 10⁵) were cultured at WillCo-dish^® glass bottom dishes (Willco Wells BV, GWST-5040) and subjected to an atomic force microscope (JPK NanoWizard 4, Bruker Nano GmbH) for imaging in QI mode. The topographical images were acquired in PBS by using a PFQNM-LC-CAL probe (Bruker Nano GmbH) with an end radius of 75 nm and a force constant around 0.09 N/m. After an entire cell was imaged by AFM in PBS, the colloid probe (MLCT-O-A probe, Bruker Nano GmbH) with a 20-μm diameter silica sphere was localized on an intact single cell, and then the mechanical stiffness (i.e., Young’s modulus) was measured by Hertz model via AFM indentation test. The Young’s modulus distributions of the lung tissue of mouse were characterized on an atomic force microscope (JPK NanoWizard 4, Bruker Nano Inc.). Frozen sections of mouse lung tissue were placed on slides. The topographies were measured by QI mode, which collected force curve matrix for further calculation of Young’s modulus distribution. A ScanAsyst-Fluid probe with a force constant around 0.7 N/m and a tip radius around 20 nm was applied in the characterization. Force curves in matrix were fitted with Hertz model to calculate the corresponding Young’s modulus by using commercial software provided from Nano Wizard 4.