Rabbit anti phospho ampkα t172

Manufactured by Cell Signaling Technology

Sourced in Japan

Rabbit anti-phospho-AMPKα T172 is a primary antibody that specifically recognizes the threonine 172 phosphorylated form of the AMPKα subunit. AMPK is a key regulator of cellular energy homeostasis.

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Lab products found in correlation

Rabbit anti raptor, by Cell Signaling Technology (1 mentions) Rabbit anti rps6, by Abcam (1 mentions) Rabbit anti cox 4, by Cell Signaling Technology (1 mentions) Peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Rabbit anti ha, by Cell Signaling Technology (1 mentions) Rabbit anti ulk1, by Cell Signaling Technology (1 mentions) Mouse anti vdac1, by Abcam (1 mentions) Rabbit anti tom20, by Cell Signaling Technology (1 mentions) Mouse anti tim23, by BD (1 mentions) Mouse anti ampk, by Abcam (1 mentions) Mouse anti mfn1, by Abcam (1 mentions) Mouse anti ndufs3, by Abcam (1 mentions) Rabbit anti flag, by Cell Signaling Technology (1 mentions) Mouse anti tubulin, by Developmental Studies Hybridoma Bank (1 mentions) Anti ampkα, by Cell Signaling Technology (1 mentions) Anti ubiquitin, by Cell Signaling Technology (1 mentions) Anti actb, by ABclonal (1 mentions) Anti smpd3, by Santa Cruz Biotechnology (1 mentions) Anti ampkα1, by Cell Signaling Technology (1 mentions) Anti ampkα2, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

AMPKα (280 citations) Anti-AMPKα (120 citations) Phospho-AMPKα (99 citations) Anti-phospho-AMPKα (48 citations) Rabbit anti-AMPKα (22 citations) Rabbit anti-phospho-AMPKα (10 citations) Phospho-AMPKα T172 (9 citations) Anti-phospho-T172 AMPKα ( (2 citations)

3 protocols using rabbit anti phospho ampkα t172

Fo-4 Western Blotting Optimization

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For western blotting of Fo-4, hyphae were treated with different concentrations of SA and total protein extracts were prepared with RIPA lysis buffer. Primary antibodies were used: rabbit anti-phospho-AMPKα T172 (#2535; Cell signaling technology), mouse anti-AMPKα (MCA2672GA; BIO-RAD), rabbit anti-phospho-RAPTOR S792 (#2083; Cell signaling technology), rabbit anti-RAPTOR (#2280; Cell signaling technology), rabbit anti-phospho-S6 ribosomal protein S235/236 (#2211; Cell signaling technology), rabbit anti-RPS6 (ab40820; Abcam), and mouse anti-yeast β-actin (AT0014; CMCTAG). The integrated optical density values of the indicated bands were quantified using the software Image-Pro Plus.

Li L., Zhu T., Song Y., Feng L., Kear P.J., Riseh R.S., Sitohy M., Datla R, & Ren M. (2021). Salicylic acid fights against Fusarium wilt by inhibiting target of rapamycin signaling pathway in Fusarium oxysporum. Journal of Advanced Research, 39, 1-13.

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Comprehensive Immunoblot Analysis of Mitochondrial Proteins

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For immunoblot analysis, the following antibodies were used: mouse anti-MFN1 (Abcam), rabbit anti-TOM20 (Cell Signaling Technology), mouse anti-TIM23 (BD Biosciences), rabbit anti-COXIV (Cell Signaling Technology), rabbit anti-UCHL1 (Cell Signaling Technology), mouse anti-tubulin (Developmental Studies Hybridoma Bank), rabbit anti–phospho-AMPKα (T172, Cell Signaling Technology), rabbit anti–phoshpo-ULK1 (S555, Cell Signaling Technology), mouse anti-AMPK (Abcam), rabbit anti-ULK1 (Cell Signaling Technology), rabbit anti-FUNDC1 (Novus Biologicals), mouse anti-VDAC1 (Abcam), mouse anti-NDUFS3 (Abcam), rabbit anti-Flag (Cell Signaling Technology), rabbit anti-HA (Cell Signaling Technology), mouse anti-Myc (MBL, Japan), mouse anti-TRIM63 (Santa Cruz Biotechnology), and rabbit anti-PKM antibody (Cell Signaling Technology). Peroxidase-conjugated secondary antibodies were purchased from the Jackson laboratory.

Ham S.J., Lee D., Xu W.J., Cho E., Choi S., Min S., Park S, & Chung J. (2021). Loss of UCHL1 rescues the defects related to Parkinson’s disease by suppressing glycolysis. Science Advances, 7(28), eabg4574.

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Antibody Validation for SMPD3 and AMPK

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Rabbit polyclonal antibody against phosphor-SMPD3-S209/208 (ABclonal, cat. #AP1312, 1:500 for IB) was raised and validated by Abclonal. Rabbit polyclonal antibody against ubiquitin-SMPD3-K103 (ABclonal, cat. #A21272, 1:500 for IB) was raised and validated by Abclonal. Rabbit anti-phospho-AMPKα-T172 (Cell Signaling Technology, cat. #2535, 1:1000 for IB), anti-AMPKα (Cell Signaling Technology, cat. #2532, 1:1000 for IB), anti-AMPKα1 (Cell Signaling Technology, cat. #2795, 1:1000 for IB) anti-AMPKα2 (Cell Signaling Technology, cat. #2757, 1:500 for IB), anti-ACTB (ABclonal, cat. #ab8227, 1:2000 for IB), anti-SMPD3 (Santa Cruz Biotechnology, cat. #sc-166637, 1:500 for IB), Anti-Ubiquitin (Medical & Biological Laboratories, cat. #MK-11-3, 1:500 for IB), Anti-Ubiquitin (linkage-specific K48) (Cell Signaling Technology, cat. #8081, 1:500 for IB), Anti-Ubiquitin (linkage-specific K63) (Cell Signaling Technology, cat. #5621, 1:500 for IB) were used in this study.

Chen B., Sun L., Zeng G., Shen Z., Wang K., Yin L., Xu F., Wang P., Ding Y., Nie Q., Wu Q., Zhang Z., Xia J., Lin J., Luo Y., Cai J., Krausz K.W., Zheng R., Xue Y., Zheng M.H., Li Y., Yu C., Gonzalez F.J, & Jiang C. (2022). Gut bacteria alleviate smoking-related NASH by degrading gut nicotine. Nature, 610(7932), 562-568.

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