Anti siglec h clone 551

Manufactured by BioLegend

Anti-Siglec H (clone 551) is a monoclonal antibody that binds to the Siglec-H antigen. Siglec-H is a sialic acid-binding immunoglobulin-like lectin expressed on plasmacytoid dendritic cells. This antibody can be used to detect and study Siglec-H-expressing cells.

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Siglec H (6 citations) Siglec-H (clone 551) (4 citations)

2 protocols using anti siglec h clone 551

Multiparameter Flow Cytometry of Murine and Human Immune Cells

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Anti-CD3 (clone 17A2, catalog # 100227), anti-CD138 (clone 281-2, catalog # 142523), anti-CD317 (clone 927, catalog # 127023), anti-I-Ab (clone M5/114.15.2, catalog # 107631), anti-IgD (clone 11-26c.2a, catalog # 405725), anti-CD4 (clone RM4-5, catalog # 100539), anti-CD5 (clone 53-7.3, catalog # 100623), anti-CD8a (clone 53-6.7, catalog # 100733), anti-CD93 (clone AA4.1, catalog # 136511), anti-IgM (clone RMM-1, catalog # 406511), anti-B220 (clone RA3-6B2, catalog # 103235), anti-F4/80 (clone BM8, catalog # 123127), anti-CD1d (clone 1B1, catalog # 123521), anti-CD11b (clone M1/70, catalog # 101211), anti-CD11c (clone N418, catalog # 117309), anti-CD19 (clone 6D5, catalog # 115512), anti-Siglec H (clone 551, catalog # 129611), anti-Blimp-1 (clone 5E7, catalog # 150003) and anti-Bcl6 (clone 7D1, catalog # 358507) Abs were purchased from BioLegend and used for flow cytometric analyses of murine cells.
Anti-CD24 (clone ML5, catalog # 311105), anti-CD19 (clone HIB19, catalog # 302229), anti-CD27 (clone M-T271, catalog # 356411), anti-CD38 (clone HB-7, catalog # 356605), anti-CD20 (clone 2H7, catalog # 302357), anti-CD3 (clone UCHT1, catalog # 300433), and anti-IgD (clone IA6-2, catalog # 348220) Abs were purchased from BioLegend and used for flow cytometric analyses of human cells.

Kunishita Y., Yoshimi R., Kamiyama R., Kishimoto D., Yoshida K., Hashimoto E., Komiya T., Sakurai N., Sugiyama Y., Kirino Y., Ozato K, & Nakajima H. (2020). TRIM21 Dysfunction Enhances Aberrant B-Cell Differentiation in Autoimmune Pathogenesis. Frontiers in Immunology, 11, 98.

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Flow Cytometric Analysis of Immune Cells

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The primary antibodies used for flow cytometry were as follows: anti‐F4/80 (clone BM8, BioLegend), anti‐CD11c (clone N418, BioLegend), anti‐MHC II (clone 10‐3.6, BioLegend), anti‐PDCA1 (clone 927, BioLegend), anti‐Gr1 (clone RB6‐8C5, BioLegend), anti‐CD4 (clone GK1.5, BioLegend), anti‐CD8a (clone 53‐6.7, BioLegend), anti‐IFN‐γ (clone XMG1.2, BioLegend), anti‐CD44 (clone IM7, BioLegend), anti‐CD80 (clone 16‐10A1, BioLegend), anti‐CD62L (clone MEL‐14, BioLegend), anti‐CD11b (clone M1/70, BioLegend), anti‐CD86 (clone GL‐1, BioLegend), anti‐I‐A/I‐E (clone M5/114.15.2, BioLegend), DC Marker (clone 33D1, BioLegend), anti‐Siglec‐H (clone 551, BioLegend), anti‐CD3ε (clone 145‐2C11, BioLegend), anti‐CD19 (clone 6D5, BioLegend), anti‐Ly‐6G (clone 1A8, BioLegend) and anti‐CD335 (clone 29A1.4, BioLegend). Mononuclear cells were isolated from the spleen and draining lymph nodes. A total of 1 × 10⁶ cells were incubated with 1·5 mg mL⁻¹ antibody for 30 min at 4°C. For intracellular staining, cells were fixed and permeabilized using fixation/permeabilization buffer (eBioscience) for 30 min at 4°C. The cells were then washed and stained for 30 min at 4°C with antibodies diluted with permeabilization buffer. The cell suspensions were analysed on an LSRII flow cytometer (BD Biosciences), and the data were analysed using FlowJo.

Geng G., Xu C., Peng N., Li Y., Liu J., Wu J., Liang J., Zhu Y, & Shi L. (2021). PTBP1 is necessary for dendritic cells to regulate T‐cell homeostasis and antitumour immunity. Immunology, 163(1), 74-85.

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