Purified origami structures were diluted to approximately 25 ng/μL prior to imaging. 5 μL of the diluted
origami was applied to glow-discharged, carbon-coated, 400-mesh formvar grids (Ted Pella) for 1.5 min. The grid was then blotted
dry on filter paper (Whatman). Washing and staining was performed by preparing a pierce of parafilm with two 15-μL droplets
of 1× folding buffer and two 15-μL droplets of 2% aqueous uranyl formate (electron microscopy sciences (EMS)) stain
solution. The grid was dipped onto the first buffer droplet, blotted dry, dipped onto the next buffer droplet, blotted dry, dipped
onto the first stain droplet, blotted dry, and then held onto the final stain droplet for 45 s before being blotted dry. Grids
were then allowed to air-dry for 10 min prior to imaging. Electron micrographs were collected using an FEI TECNAI T12 transmission
electron microscope using a using a 4k × 4k charge-coupled device camera (UltraScan 4000, Gatan) at 26 000× and 52
000× magnifications. Class averages were obtained using EMAN2 software.
origami was applied to glow-discharged, carbon-coated, 400-mesh formvar grids (Ted Pella) for 1.5 min. The grid was then blotted
dry on filter paper (Whatman). Washing and staining was performed by preparing a pierce of parafilm with two 15-μL droplets
of 1× folding buffer and two 15-μL droplets of 2% aqueous uranyl formate (electron microscopy sciences (EMS)) stain
solution. The grid was dipped onto the first buffer droplet, blotted dry, dipped onto the next buffer droplet, blotted dry, dipped
onto the first stain droplet, blotted dry, and then held onto the final stain droplet for 45 s before being blotted dry. Grids
were then allowed to air-dry for 10 min prior to imaging. Electron micrographs were collected using an FEI TECNAI T12 transmission
electron microscope using a using a 4k × 4k charge-coupled device camera (UltraScan 4000, Gatan) at 26 000× and 52
000× magnifications. Class averages were obtained using EMAN2 software.