Stat1 sirna

Manufactured by Thermo Fisher Scientific

STAT1 siRNA is a small interfering RNA designed to target and silence the STAT1 gene. It is a tool for molecular biology research, allowing for the investigation of STAT1 gene function and its role in various cellular processes.

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Lab products found in correlation

Stat3 sirna, by Thermo Fisher Scientific (2 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Irf1 sirna, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

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SiRNAs (258 citations) STAT1 (9 citations)

3 protocols using stat1 sirna

Silencing STAT1 Gene Expression

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Cells were transfected at 30-40% confluence with 10 nM STAT1 or control siRNA with Lipofectamine RNAiMAX and Opti-MEM (Life Technologies) according to the manufacturer’s instructions. Cells were incubated in antibiotic- and serum-free IMDM Glutamax with siRNA for 48 hours, then rinsed with PBS prior to drug treatments. STAT1 siRNA from Ambion (siRNA ID #s277) was used with the following sequences:
5’ – CCUACGAACAUGACCCUAUtt – 3’ (sense)
5’ – AUAGGGUCAUGUUCGUAGGtg – 3’ (antisense)
For verification, a second STAT1 siRNA from Ambion (siRNA ID #s279) was used with the following sequences:
5’ – GGUUCACUAUAGUUGCGGAtt – 3’ (sense)
5’ – UCCGCAACUAUAGUGAACCag – 3’ (antisense)

Schmitt N.C., Trivedi S, & Ferris R.L. (2015). STAT1 Activation is Enhanced by Cisplatin and Variably Affected by EGFR Inhibition in HNSCC Cells. Molecular cancer therapeutics, 14(9), 2103-2111.

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Evaluating Melanoma Cell Signaling Pathways

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UACC1273 melanoma cells were transfected with indicated siRNAs (10pM) or
scramble (Scr) siRNA (10pM) with Lipofectamine RNAiMAX Reagent (Invitrogen) according to
the manufacturer’s protocol. Transfection efficiency was assessed by measuring the
amounts of the proteins of interest. The siRNAs are listed below: Scramble siRNA (Scr,
Silencer® Select Negative Control No. 1 siRNA, Catalog number: 4390843, Ambion);
STAT1 siRNA S1-1, sense, 5′-CGGUUGAACCCUACACGAATT-3′, ID:s278, Ambion;
STAT1 siRNA S1-2, sense, 5′-CCUACGAACAUGACCCUAUTT -3′, ID:s277, Ambion;
STAT3 siRNA S3-1, sense, 5′- GCACCUUCCUGCUAAGAUUtt-3′, ID:s745, Ambion;
STAT3 siRNA S3-2, sense, 5′-GCCUCAAGAUUGACCUAGATT -3′, ID:s743, Ambion;
IRF1 siRNA IRF1-1, sense, 5′-GCAGAUUAAUUCCAACCAATT -3′, ID:s7502, Ambion;
IRF1 siRNA IRF1-2, sense, 5′- CCUCUGAAGCUACAACAGATT-3′, ID:s7501, Ambion.
All siRNAs were purchased from Thermo Scientific.

Mo X., Zhang H., Preston S., Martin K., Zhou B., Vadalia N., Gamero A.M., Soboloff J., Tempera I, & Zaidi M.R. (2017). Interferon-γ signaling in melanocytes and melanoma cells regulates expression of CTLA-4. Cancer research, 78(2), 436-450.

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Interrogating IFN-γ Signaling Pathways

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The contributions of STAT1, SOCS1 and STAT3 to changes in AGT expression by IFN-γ treatment were examined using small interference RNA (siRNA) technology as previously described.^{27 (link)} In brief, MCs were plated on six-well plates with STAT1-siRNA (Ambion; sense sequence; 5′–CCUUUGUGGUGGAACGACATT–3′), SOCS1-siRNA (Ambion; sense sequence; 5′–CGAGCAUUCGUGUGCACUUTT–3′) or STAT3-siRNA (Ambion; sense sequence; 5′–GCAGAGUUCAAGCACCUGATT–3′). Treatment with the transfection reagent (Lipofectamine 2000; Invitrogen) alone did not show a difference in basal SOCS1 and basal AGT and MCP-1 expression levels compared with negative control siRNA (Ambion)-transfected MCs; therefore, the transfection reagent alone (siRNA (–) group) was used as control groups in the these experiments. Cells were harvested after 48 hours transfection with siRNA to ensure suppression of STAT1 and SOCS1 protein using western blot analysis. At this time point, cells were treated with IFN-γ to evaluate the roles of STAT1, SOCS1 and STAT3 in the regulation of AGT and MCP-1 expression.

Penrose H.M., Katsurada A., Miyata K., Urushihara M, & Satou R. (2020). STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells. Journal of the Renin-Angiotensin-Aldosterone System: JRAAS, 21(3), 1470320320946527.

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