Tissue extracts (50 μg per lane) were prepared in lysis buffer [10 mM tris-HCl (pH 8), 150 mM NaCl, 1% Triton X-100, and protease inhibitors and PhosSTOP (Roche)] and subjected to Western blotting. The Western blot was probed with anti-p-AMPK and anti-AMPK, p-ACC and ACC, p-mTOR and mTOR, p-S6 and S6 (Cell Signaling), and tubulin antibodies. Immunodetection with an appropriate secondary peroxidase-conjugated antibody (DAKO) was followed by chemiluminescence (Amersham). Protein band quantification was performed using LabImage software.
P mtor and mtor
Manufactured by Cell Signaling Technology
P-mTOR and mTOR are antibodies used in laboratory research to detect and quantify the phosphorylated and total forms of the mTOR protein, respectively. mTOR is a serine/threonine protein kinase that plays a central role in regulating cell growth, proliferation, motility, survival, protein synthesis, and transcription. These antibodies are useful tools for studying the activity and regulation of the mTOR signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Peroxidase conjugated secondary antibody, by Agilent Technologies (1 mentions)
Anti atg12, by Santa Cruz Biotechnology (1 mentions)
Anti sirt1, by Cell Signaling Technology (1 mentions)
Anti parkin, by Cell Signaling Technology (1 mentions)
Immun star hrp substrate kit, by Bio-Rad (1 mentions)
Anti bcl 2, by Santa Cruz Biotechnology (1 mentions)
Ppi3k and pi3k, by Cell Signaling Technology (1 mentions)
Complete protease inhibitor cocktail, by Roche (1 mentions)
Anti active caspase 3, by Cell Signaling Technology (1 mentions)
Monoclonal antibodies specific for beclin 1 and p62, by Merck Group (1 mentions)
Anti map lc3, by Santa Cruz Biotechnology (1 mentions)
Anti bax, by Santa Cruz Biotechnology (1 mentions)
2 protocols using p mtor and mtor
1
Western Blot Analysis of Signaling Proteins
2
Antibody Detection for Autophagy Markers
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Monoclonal antibodies specific for Beclin‐1 and p62 were purchased from Sigma‐Aldrich. The anti‐GAPDH monoclonal antibody was acquired from Calbiochem‐Merck Chemicals Ltd. Similarly, anti‐active caspase‐3, anti‐SIRT‐1, p‐PI3K and PI3K, p‐mTOR and mTOR, and anti‐Parkin were obtained from Cell Signaling Technology. Finally, anti‐Bcl‐2, anti‐Bax, anti‐ATG12, and anti‐MAP‐LC3 antibodies were obtained from Santa Cruz Biotechnology. A cocktail of protease inhibitors (Complete™ Protease Inhibitor Cocktail) was purchased from Boehringer Mannheim. The Immun Star HRP substrate kit was obtained from Bio‐Rad Laboratories Inc.
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