The zinc ion-dependent DNAzyme and its substrate were both synthesized by Sangon Biotech Co., Ltd. (Shanghai, China) and purified by high-performance liquid chromatography (HPLC). Their sequences were as following: DNAzyme (Enz) (5'-Biotin-(A)9CATCTCTTCTCCGAGCCGGTCGAAATAGTGAGT-FAM-3'), substrate (Sub) (5'-Cy3-ACTCACTATrAGGAAGAGATG-BHQ2-3') and cleaved substrates (CS1: 5'-Cy3-ACTCACTATrA-3', CS2: 5'-GGAAGAGATG-BHQ2-3'). Biotin labeled MUC1 antibody, biotin labeled EpCAM antibody, HRP labeled EpCAM antibody, the primary antibodies of MUC1, EpCAM and GAPDH, the second antibody goat anti rabbit IgG (HRP) were obtained from Abcam Co., Ltd. (Cambridge, MA, USA). Streptavidin, ZnCl2 and o-phenylenediamine dihydrochloride (OPD) were purchased from Sigma Aldrich (St. Louis, MO, USA). Other chemicals were all of analytical grade and used without further purification. MTT Cell Proliferation and Cytotoxicity Detection Kit, Cell Apoptosis Detection Kit with Annexin V keyfluor647 & PI and cell culture products were purchased from KeyGen Biotech. Co., Ltd. (Nanjing, China). Fetal bovine serum (FBS) was obtained from GIBCO Co., Ltd. (Grand Island, NY, USA). Deionized water was obtained through Milli-Q purification system (Millipore, Milford, MA, USA) to a resistivity of > 18 MΩ•cm.
Dnazyme
Manufactured by Sangon
Sourced in China
DNAzyme is a laboratory equipment that functions as a catalytic DNA molecule. It is capable of performing various enzymatic activities, including cleavage, ligation, and modification of RNA or DNA substrates. DNAzyme's core function is to serve as a versatile tool for applications in molecular biology, biochemistry, and biotechnology.
Automatically generated - may contain errors
Lab products found in correlation
Milli q purification system, by Merck Group (1 mentions)
Zncl2, by Merck Group (1 mentions)
O phenylenediamine dihydrochloride opd, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mtt cell proliferation and cytotoxicity detection kit, by Keygen Biotech (1 mentions)
Streptavidin, by Merck Group (1 mentions)
Dntp mix, by Solarbio (1 mentions)
Nh4 2so4, by Sangon (1 mentions)
Phi29 dna polymerase, by Sangon (1 mentions)
Bovine serum albumin (bsa), by Sangon (1 mentions)
T4 dna ligase, by Sangon (1 mentions)
2 protocols using dnazyme
1
Zinc Ion-Dependent DNAzyme Assay
2
Ultrasensitive microRNA Detection Protocol
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The microRNAs, the padlock probe and DNAzyme, were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). T4 DNA ligase, phi29 DNA polymerase, exonuclease I (EXO I), ribonuclease inhibitor, BSA, (NH4)2SO4, and Tris-buffer solution (1 mol L−1; pH 8.0) were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). dNTP mix (25 mmol L−1 each) was purchased from Solarbio. Cell culture-grade ultrapure water was purchased from KeyGEN BioTECH. Magnesium chloride was purchased from Shanghai Macklin Biochemical industry (Shanghai, China). The sequences of nucleic acids employed in this study are given in Table 1 in the Supporting Information.
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