After 10 d of culture under inducing conditions, 2 × 106 BM-EPCs were incubated with the FcR blocking reagent (Miltenyi Biotec Inc., Auburn, CA, United States) and fluorescein isothiocyanate (FITC)-conjugated rabbit anti-rat CD133 antibody (BD Biosciences) and rabbit anti-rat vascular endothelial growth factor receptor 2 (VEGFR2) antibody (BD Biosciences) for 30 min at 4 °C, respectively. Then, cells were incubated with phycoerythrin (PE)-conjugated goat anti-rabbit secondary antibody (BD Biosciences) for 30 min at 4 °C. The phenotypic expression of BM-EPCs was analyzed by flow cytometry (FACS Scan flow cytometer; BD). BMMCs (2 × 106) cultured without induction for 10 d were used as a control.
Vegfr2 antibody
Manufactured by BD
Sourced in United States
The VEGFR2 antibody is a laboratory reagent used for the detection and study of the vascular endothelial growth factor receptor 2 (VEGFR2) protein. VEGFR2 is a receptor tyrosine kinase that plays a crucial role in angiogenesis, the process of new blood vessel formation. The antibody can be used in various applications, such as Western blotting, immunohistochemistry, and flow cytometry, to identify and quantify the expression of VEGFR2 in biological samples.
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Lab products found in correlation
2 protocols using vegfr2 antibody
1
Phenotypic Analysis of Bone Marrow-Derived Endothelial Progenitor Cells
2
Characterization of Bone Marrow-Derived Endothelial Progenitor Cells
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After 10 days of culture under inducing conditions, 2 × 106 BM-EPCs
were incubated with the FcR blocking reagent (Miltenyi Biotec Inc., Auburn, CA,
USA), the fluorescein isothiocyanate-conjugated rabbit anti-rat CD34 antibody
(BD Biosciences), and the rabbit anti-rat vascular endothelial growth factor
receptor 2 (VEGFR2) antibody (BD Biosciences) for 30 min at 4°C. Then, cells
were incubated with phycoerythrin-conjugated goat anti-rabbit secondary antibody
(BD Biosciences) for 30 min at 4°C. The phenotypic expression of BM-EPCs was
analyzed by flow cytometry (FACS Scan flow cytometer; BD). BMSCs (2 ×
106) cultured without induction for 10 days were used as a
control.
were incubated with the FcR blocking reagent (Miltenyi Biotec Inc., Auburn, CA,
USA), the fluorescein isothiocyanate-conjugated rabbit anti-rat CD34 antibody
(BD Biosciences), and the rabbit anti-rat vascular endothelial growth factor
receptor 2 (VEGFR2) antibody (BD Biosciences) for 30 min at 4°C. Then, cells
were incubated with phycoerythrin-conjugated goat anti-rabbit secondary antibody
(BD Biosciences) for 30 min at 4°C. The phenotypic expression of BM-EPCs was
analyzed by flow cytometry (FACS Scan flow cytometer; BD). BMSCs (2 ×
106) cultured without induction for 10 days were used as a
control.
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