Hanks balanced salt solution hbss

Lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, and glutaraldehyde were purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). Caprylic acid, capric acid, linoleic acid, SLS, sodium chloride (NaCl), disodium hydrogen phosphate 12-water (Na₂HPO₄•12H₂O), potassium dihydrogen phosphate (KH₂PO₄), sodium chloride (KCl), potassium hydroxide (KOH), hydrochloric acid (HCl), manganese (II) chloride tetrahydrate (MnCl₂•4H₂O), magnesium sulfate heptahydrate (MgSO₄•7H₂O), iron (II) sulfate heptahydrate (FeSO₄•7H₂O), calcium chloride dihydrate (CaCl₂•2H₂O), agar, crystal violet, Hank’s balanced salt solution (HBSS)(+) and 99.5% ethanol (EtOH) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). SLES was purchased from the NOF Corporation (Tokyo, Japan). The Dulbecco’s phosphate-buffered saline (d-PBS)(+) preparation reagent (with Ca and Mg) (100×) and fetal bovine serum (FBS) were purchased from Nacalai Tesque (Kyoto, Japan) and Thermo Fisher Scientific K.K. (Tokyo, Japan), respectively. WELPAS^® antiseptic solution for hands (0.2%), an alcohol-based disinfectant, was purchased from Maruishi Pharmaceutical Co. Ltd. (Osaka, Japan).

MEM essential amino acids solution, MEM non-essential amino acids solution, l-cystine, l-arginine, l-histidine, l-Isoleucine, l-leucine, l-lysine, l-methionine, l-phenylalanine, l-threonine, l-tryptophan, l-tyrosine, l-valine, William's Medium E (WME), Dulbecco's modified Eagle's medium (DMEM), and hanks' balanced salt solution (HBSS), were purchased from FUJIFILM Wako Pure Chemical Corporation (Tokyo, Japan). Proteinase inhibitor cocktail was purchased from Nacalai Tesque, (Kyoto, Japan). Na₂S₄ was purchased from Dojindo Molecular Technologies, Inc (Kumamoto, Japan). β-(4-hydroxyphenyl)ethyl iodoacetamide (HPE-IAM) was purchased from Molecular Biosciences (CO, USA). All other reagents and chemicals were of the highest grades available.