In order to measure cecal lumenal short chain fatty acids, a sample of cecal content was weighed and stored at −80 °C until analyzed. Samples and serially diluted standards were acidified using 6N HCl (1 volume of HCl to 5 volume of diluted sample) in the presence of 400 μM acetic acid-d4 and valeric acid (internal standards), then extracted with equal volume of ether. Solid samples (cecal content, fecal pellet) were lysed using a Qiagen TissueLyser II. The ether fraction was derivatized by MTBSTFA (1 to 10 volume of ether fraction) at room temperature for greater than 24 hrs, and used for GCMS for detection. Samples were run on Agilent Technologies 5977A GC/MSD system and short chain fatty acids were normalized to wet weight. For the succinate quantification in the cecal content, snap frozen samples were processed using a colorimetric succinate assay kit (Abcam, ab204718) according to the manufacturer’s instructions, including deproteinization and decolorization.
5977a gc msd system
Manufactured by Agilent Technologies
The 5977A GC/MSD system is a gas chromatography-mass spectrometry (GC/MS) instrument designed for analytical applications. It combines gas chromatography for sample separation with mass spectrometry for compound identification and quantification. The system is capable of analyzing a wide range of volatile and semi-volatile organic compounds.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 5977a gc msd system
1
Quantitative Analysis of Cecal Short-Chain Fatty Acids
2
GC-MS Analysis of Volatile Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The GC-MS analysis was carried out with an Agilent 5977A GC-MSD system. The system was equipped with an automatic liquid sampler (Agilent 7693). HP-5MS 5% capillary column (coated with methyl silicone) (30 m × 250 μm × I.D., 0.25 μm) was used as the stationary phase. The temperature was programmed from 60 to 325 °C at a rate of 1°C/minute. The injector and interface temperatures were maintained at and 350 °C, respectively. Mass spectra were taken at 70 eV. Mass range was from m/z 10 to 600.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!