P ikb α

Manufactured by GeneTex

Sourced in United States

P-IKB-α is a primary antibody that recognizes the phosphorylated form of the inhibitor of NF-kappa-B alpha (IKB-α) protein. IKB-α is a critical regulator of the NF-kappa-B signaling pathway, and its phosphorylation is a key step in the activation of this pathway.

Automatically generated - may contain errors

Lab products found in correlation

Af5103, by Affinity Biosciences (1 mentions) Af0639, by Affinity Biosciences (1 mentions) Af7018, by Affinity Biosciences (1 mentions) Af5418, by Affinity Biosciences (1 mentions) Af5393, by Affinity Biosciences (1 mentions) Confocal fluorescence microscopy, by Olympus (1 mentions) P nf κb p65, by Abcam (1 mentions) Anti lc3b, by Cell Signaling Technology (1 mentions)

2 protocols using p ikb α

Western Blot Analysis of NF-κB Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were harvested and lysed in ice-cold RIPA (Radio-Immunoprecipitation Assay) buffer mixed with cocktail and PMSF for 30 min. The BCA protein assay kit (BB-3401, Shanghai Beibo Biotechnology, Shanghai, China) was used to measure the protein concentration. Then, equal amounts of protein were resolved by 7.5% to 12.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto polyvinylidene fluoride (PVDF) membranes.
After blocked with 5% skim milk for 2 h at room temperature, the membranes were incubated with appropriate primary antibodies against p-NF-κB p65 (CST, #3033S, 1:1000), NF-κB p65 (CST, #8242S, 1:1000), p-IKB-α (GeneTex, #32224, 1:1000), IKB-α (GeneTex, #110521, 1:1000), NLRP3 (Abcam, #ab263889, 1:1000), ASC (Affinity, #DF6304, 1:500), IL-1β (Affinity, #AF5103, 1:1000), caspase-1 (Affinity, #AF5418, 1:1000), Nrf2 (Affinity, #AF0639, 1:1000), HO-1 (Affinity, #AF5393, 1:1000) and β-actin (Affinity, #AF7018, 1:1000) overnight at 4°C. Subsequently, membranes were incubated with secondary HRP-conjugated goat anti-rabbit IgG (Immunoglobulin G, Affinity, #072102, 1:5000) for 1 h. Protein bands were visualized using the enhanced chemiluminescence (ECL) (Tanon 4200SF, China) detection system. The intensity of the bands was assessed using the ImageJ software (National Institutes of Health, Bethesda, MA, USA) tool.

Cheng J.J., Ma X.D., Ai G.X., Yu Q.X., Chen X.Y., Yan F., Li Y.C., Xie J.H., Su Z.R, & Xie Q.F. (2022). Palmatine Protects Against MSU-Induced Gouty Arthritis via Regulating the NF-κB/NLRP3 and Nrf2 Pathways. Drug Design, Development and Therapy, 16, 2119-2132.

+ Open protocol

+ Expand

Immunofluorescence Analysis of Autophagy and NF-κB

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were washed three times with phosphate-buffered saline (PBS), fixed with 4% paraformaldehyde for 15 minutes, and blocked with bovine serum albumin (BSA) at 37°C for 30 minutes. After washing three times with PBS, the cells were incubated with anti-LC3B (1:100, Cell Signaling Technology, Danvers, MA, USA), p-IKBα (1:200, GeneTex, USA) and p-NF-κBp65 (1:1000, Abcam), overnight at 4°C, followed by goat anti-rabbit antibody (1:200 Zhongshan Golden Bridge, Beijing, China) for 1 hour at 37°C in the dark. The cells were counterstained with DAPI (Yusen Biotech Inc, Shanghai, China) for 1 hour. Finally, the positive cells was analyzed by confocal fluorescence microscopy (Olympus Tokyo, Japan).

Liu L., Wang Y., Geng C., Wang A., Han S., You X., Sun Y., Zhang J., Lu W, & Zhang Y. (2021). CD155 Promotes the Progression of Cervical Cancer Cells Through AKT/mTOR and NF-κB Pathways. Frontiers in Oncology, 11, 655302.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!