2 2 azobis 2 methyl n 2 hydroxyethyl propionamide va 086

The purified sulfated polysaccharide (AdSP, polysaccharide content of ~99%, Mw = 272 kDa) extracted from self-grown Arthrospira maxima through the pressurized hot water extraction method was provided by Far East Bio-Tec. Co., Ltd. (Taipei, Taiwan). The molar ratio of each monosaccharide composition is listed in Table 2. Methyl acrylic anhydride (MAA), gelatin (type A, 300 Bloom, from porcine skin), deuterium oxide (D₂O), and sodium bicarbonate were purchased from Sigma-Aldrich (St. Louis, MO, USA). High-glucose Dulbecco’s Modified Eagle’s Medium (HG-DMEM), Ham’s F-12, fetal bovine serum (FBS), and penicillin–streptomycin–amphotericin (PSA) were purchased from Gibco (Grand Island, NY, USA). Geneticin (G418) was purchased from Invitrogen (Waltham, MA, USA). 2,2-Azobis(2-methyl-N-(2-hydroxyethyl) propionamide) (VA-086) and FG were purchased from Wako Chemicals GmbH (Neuss, Germany). Chitosan (Mn~1.4 × 10⁵ Da) was obtained from Hopax lnc. (Taipei, Taiwan). CCK-8 (Sigma-Aldrich, St. Louis, MO, USA) and KAPA SYBR Green qPCR kit (Kapa Biosystems, Inc., Wilmington, MA, USA) were applied in the commercial form.

Sublimed sulfur powder,
lithium nitrate (LiNO₃), dopamine hydrochloride, potassium
ferricyanide (III), anhydrous
ethanol, bis(trifluoromethane)sulfonimide lithium salt (LiTFSI), polyvinylidene
fluoride (PVDF), N-methyl-2-pyrrolidone (NMP), 1-vinylimidazole
(≥99%), 1-bromodecane (98%), 1,2-dimethoxyethane (DME), carbon
disulfide, tris(hydroxymethyl) aminomethane (Tris), and 1,3-dioxolane
(DOL) were purchased from Sigma-Aldrich. Hydrochloric acid solution
(37%) and melamine were purchased from Alfa-Aesar. Water-soluble nonionic
azo initiator 2,2′-azobis[2-methyl-N-(2-hydroxyethyl)
propionamide] (VA086) was obtained from Wako Chemicals. All chemicals
were used without any further purification.