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β actin monoclonal antibody

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The β-actin monoclonal antibody is a laboratory reagent used to detect the presence and quantify the expression levels of the β-actin protein in biological samples. β-actin is a widely expressed cytoskeletal protein that is often used as a reference or housekeeping gene for normalization in various molecular biology techniques.

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Lab products found in correlation

Sarnp polyclonal antibody, by Thermo Fisher Scientific (2 mentions) Sc35 monoclonal antibody, by Merck Group (2 mentions) Son polyclonal antibody, by GeneTex (2 mentions) Ripa lysate, by Beyotime (1 mentions) Bcl2 monoclonal antibody, by Thermo Fisher Scientific (1 mentions) Inverted fluorescence microscope, by Thermo Fisher Scientific (1 mentions) Pipettor, by Eppendorf (1 mentions) Transwell chamber, by Merck Group (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Paclitaxel, by Merck Group (1 mentions) Ly294002, by Merck Group (1 mentions) Annexin 5 fitc apoptosis assay kit, by BD (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Temed, by Thermo Fisher Scientific (1 mentions) Sodium dodecyl sulfate (sds), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions)

6 protocols using β actin monoclonal antibody

1

Antibody Dilutions for Immunofluorescence and Western Blot

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SARNP polyclonal antibody (Invitrogen, PA5–56586) was used at a 1:250 dilution in immunofluorescence assay (IFA) and 1:1000 dilution for Western blot assay. β-actin monoclonal antibody (Invitrogen AM4302) was used at 1:5000 dilution in Western blot. SC35 monoclonal antibody (Sigma Aldrich SAB4200725) was used at 1:500 dilution for IFA. SON polyclonal antibody (Genetex GTX129778) was used at 1:500 dilution for IFA.
Xie Y., Gao S., Zhang K., Bhat P., Clarke B.P., Batten K., Mei M., Gazzara M., Shay J.W., Lynch K.W., Angelos A.E., Hill P.S., Ivey A.L., Fontoura B.M, & Ren Y. (2023). Structural basis for high-order complex of SARNP and DDX39B to facilitate mRNP assembly. Cell reports, 42(8), 112988.
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2

Antibody Dilutions for Immunofluorescence and Western Blot

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SARNP polyclonal antibody (Invitrogen, PA5–56586) was used at a 1:250 dilution in immunofluorescence assay (IFA) and 1:1000 dilution for Western blot assay. β-actin monoclonal antibody (Invitrogen AM4302) was used at 1:5000 dilution in Western blot. SC35 monoclonal antibody (Sigma Aldrich SAB4200725) was used at 1:500 dilution for IFA. SON polyclonal antibody (Genetex GTX129778) was used at 1:500 dilution for IFA.
Xie Y., Gao S., Zhang K., Bhat P., Clarke B.P., Batten K., Mei M., Gazzara M., Shay J.W., Lynch K.W., Angelos A.E., Hill P.S., Ivey A.L., Fontoura B.M, & Ren Y. (2023). Structural basis for high-order complex of SARNP and DDX39B to facilitate mRNP assembly. Cell reports, 42(8), 112988.
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3

Liver Protein Extraction and Western Blot

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The liver tissue was homogenized lysis buffer (1% Triton X-100, 20 mM Tris, 40 mM NaF, 0.2% SDS, 0.5% deoxycholate, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 100 mM NaCl, pH 7.5). The extract was centrifuged at 15,000× g for 15 min at 4 °C and then stored at −20 °C before use. Target protein in liver tissue sample was applied for Western blot according to the previous studies [40 (link),41 (link)]. The samples were separated on 10% SDS-PAGE gels and then transferred to polyvinylidene fluoride membranes. PPAR-α polyclonal antibody (sc-398394, Santa Cruz Biotechnology Inc., Dallas, TX, USA), acetyl-CoA carboxylase (ACC) monoclonal antibody (3676, Cell Signaling Technology Inc., Danvers, MA, USA), β-actin monoclonal antibody (MA5-15739, Thermo Fisher Scientific, Rockford, IL, USA) were used to determine the protein expression of PPAR-α, ACC, and β-actin by using immunoblotting.
Lin T.A., Ke B.J., Cheng S.C, & Lee C.L. (2021). Red Quinoa Bran Extract Prevented Alcoholic Fatty Liver Disease via Increasing Antioxidative System and Repressing Fatty Acid Synthesis Factors in Mice Fed Alcohol Liquid Diet. Molecules, 26(22), 6973.
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4

UVB-Induced Apoptosis Pathway Analysis

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Photolyase was applied to the cells for 2 h, followed by UVB irradiation for 2 min and 15 s and culturing for another 6 h. Cell lysates were collected using RIPA lysates (Beyotime, Shanghai, China). Primary antibodies were incubated overnight at 4 °C, followed by secondary antibodies for 1 h at room temperature. The following antibodies were used: BCL-2 monoclonal antibody (ThermoFisher Scientific, Cambridge, MA, USA); Bax monoclonal antibody (ThermoFisher Scientific; USA33-6400); caspase 3 monoclonal antibody (ThermoFisher Scientific, 700182); and β-Actin monoclonal antibody (ThermoFisher Scientific, MA1-140).
Wang Z., Li Z., Lei Y., Liu Y., Feng Y., Chen D., Ma S., Xiao Z., Hu M., Deng J., Wang Y., Zhang Q., Huang Y, & Yang Y. (2022). Recombinant Photolyase-Thymine Alleviated UVB-Induced Photodamage in Mice by Repairing CPD Photoproducts and Ameliorating Oxidative Stress. Antioxidants, 11(12), 2312.
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5

Investigating PI3K/Akt Pathway in Lung Cancer

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Lung cancer cell line NCI-H446 (Kunming Cell Bank, Chinese Academy of Sciences); methyl thiazolyltetrazolium (MTT), dimethylsulfoxide (DMSO), paclitaxel, lobaplatin and LY294002 (Sigma, St. Louis, MO, USA); RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA); rabbit anti-human PI3K, p-Akt, AKT, phosphorylated-glycogen synthase kinase 3β (p-GSK3β), GSK3β, β-actin monoclonal antibody and goat anti-rabbit secondary polyclonal antibody (cat. nos. 4249, 4060, 4685, 9323, 9315, 4970 and 14708) (Cell Signaling Technology, Danvers, MA, USA); Annexin V-FITC apoptosis assay kit (BD Biosciences, Heidelberg, Germany); inverted fluorescence microscope (Thermo Fisher Scientific, Inc., Waltham, MA, USA); cell culture bottle (Corning Inc., Corning, NY, USA); Transwell chamber (EMD Millipore, Billerica, MA, USA) and pipettor (Eppendorf AG, Hamburg, Germany).
Ma D., Li S., Cui Y., Li L., Liu H., Chen Y, & Zhou X. (2018). Paclitaxel increases the sensitivity of lung cancer cells to lobaplatin via PI3K/Akt pathway. Oncology Letters, 15(5), 6211-6216.
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6

Apoptosis-related Protein Expression

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RPMI1640, DMEM (Dulbecco's Modified Eagle's Medium) culture media and fetal bovine serum were purchased from GIBCO; Parenzyme, SDS, TEMED, β-actin monoclonal antibody, HA monoclonal antibody (Cat. # TA-04), Bcl-2 monoclonal antibody (sc-783) and Bax monoclonal antibody (sc-526) were purchased from Santa Cruz Biotechnology; PUMA polyclonal antibody (Cat. #55120-1-AP) was purchased from USA Proteintech; IgG (ZDR-5307) and IgG (ZDR-5306) were purchased from Beijing ZSGB-Bio Company; DDP cisplatin was purchased from Shandong Qilu Pharmaceutical Co., Ltd.
Liu C.J., Zhang X.L., Luo D.Y., Zhu W.F., Wan H.F., Yang J.P., Yang X.J, & Wan F.S. (2015). Exogenous p53 upregulated modulator of apoptosis (PUMA) decreases growth of lung cancer A549 cells. Asian Pacific journal of cancer prevention : APJCP, 16(2).
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