UPLC analysis performed on an Agilent 1290 Infinity LC HPLC system (Agilent Technologies, Wilmington, USA). Chromatographic separation was acquired on Waters T3 C18 column (100 × 2.1 mm, 1.8 μm; C18 MWM-13). The detection conditions were as follows: wavelength, 347 nm; flow rate, 0.35 mL/min; column temperature, 40 ℃; sample volume, 2 μL. The elution solution was water comprised 0.04% formic acid and acetonitrile. And the gradient solution procedure for acetonitrile was: 0.0~10.0 min: 95~5%; 10.0~11.0 min: 5~5%; 11.1~14.0 min: 95~95%.
Agilent 1290 infinity lc hplc system
Manufactured by Agilent Technologies
Sourced in United States, Germany
The Agilent 1290 Infinity LC HPLC system is a high-performance liquid chromatography (HPLC) instrument designed for analytical applications. The system includes a solvent delivery system, an autosampler, a column compartment, and a detector. It is capable of performing high-resolution separations and provides accurate and reliable data for a wide range of analytical tasks.
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2 protocols using agilent 1290 infinity lc hplc system
1
UPLC Profiling of Bioactive Compounds
2
Glycerol Quantification via HPLC
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For glycerol determination, samples were retrieved at specific times and centrifuged at 4 °C and 16,000 × g for 5 min The resulting supernatant was then filtered through a 0.22 μm filter (Millipore) for subsequent HPLC analysis onto an Agilent 1290 Infinity LC HPLC system (Waldbronn, Germany) coupled with a Refractive Index Detector (RID) (Agilent 1260 Infinity). Compound separation was achieved using a Hi-Plex H ion-exchange analytical column (Agilent, Santa Clara, CA, USA) with a 7.7 × 300 mm and 8 μm pore size. The mobile phase consisted of a 5 mM H2SO4 solution prepared with ultrapure water, filtered through a 0.2 μm pore membrane and degassed for 15 min before use. Flow rate was set to 0.6 mL/min and column temperature was set to 65 °C.
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